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  4. Recombinant Saccharomyces cerevisiae Myosin-2 (MYO2), partial

Recombinant Saccharomyces cerevisiae Myosin-2 (MYO2), partial

  • 中文名稱：
    釀酒酵母MYO2重組蛋白
  • 貨號：
    CSB-YP015296SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母MYO2重組蛋白
  • 貨號：
    CSB-EP015296SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母MYO2重組蛋白
  • 貨號：
    CSB-EP015296SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母MYO2重組蛋白
  • 貨號：
    CSB-BP015296SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母MYO2重組蛋白
  • 貨號：
    CSB-MP015296SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MYO2
  • Uniprot No.：
  • 別名：
    MYO2; CDC66; YOR326W; O6167; Myosin-2; Cell division control protein 66; Class V unconventional myosin MYO2; Type V myosin heavy chain MYO2; Myosin V MYO2
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Myosin heavy chain that is required for the cell cycle-regulated transport of various organelles and proteins for their segregation. Functions by binding with its tail domain to receptor proteins on organelles and exerting force with its N-terminal motor domain against actin filaments, thereby transporting its cargo along polarized actin cables. Essential for the delivery of secretory vesicles to sites of active growth during bud emergence and cytokinesis. Required for segregation and inheritance of peroxisomes, late Golgi compartments, mitochondria and the vacuole to the daughter cell during cell division. Also required for correct alignment of the spindle during mitosis.
  • 基因功能參考文獻：
    1. spatial and temporal regulation of myosin V in vivo by a head-to-tail interaction is critical for the normal delivery functions of the motor. PMID: 25940346
    2. These findings reveal a new fast-acting energy conservation strategy halting growth by immobilizing myosin V in a newly described state on selectively stabilized actin cables. PMID: 25308080
    3. myosin-V is activated by binding a secretory vesicle and that myosin-V mutations that compromise vesicle binding render the motor constitutively active PMID: 23079598
    4. Myo2p is not processive on bare actin but highly processive on actin-tropomyosin. Tpm1p supports the most robust processivity. PMID: 22704989
    5. Myo2p interacts with Pex19p PMID: 22486971
    6. It was shown that Mmr1 functions as a mitochondria-specific cargo adaptor early in the cell cycle and that Mmr1 binds Myo2 at the site that binds Vac17. PMID: 22753895
    7. Yeast myosin-II (Myo1p) and myosin-V (Myo2p) accommodate the reduced N-terminal charge density of yeast actin, showing greater activity with yeast over muscle actin PMID: 21757693
    8. Myo2 is an essential and direct mediator of bud-directed mitochondrial movement in yeast. PMID: 21807878
    9. During polarization, cells change from fast to slow cable dynamics through spatio-temporal regulation of Bni1, Bnr1 and Myo2. PMID: 21486946
    10. Results characterize the interaction between Sro7 and the type V myosin Myo2 and show that this interaction is important for two distinct aspects of Sro7 function. PMID: 21248204
    11. retention of mitochondria in the bud was compromised in YPT11 and MYO2 mutants PMID: 15215313
    12. The globular tail of Myo2p contains two subdomains (I and II) that have distinct functions, vacuole-specific and secretory vesicle-specific movement. Subdomain I tightly associates with subdomain II. PMID: 15684027
    13. Myo2p is subject to phosphoregulation involving a PKA-related signaling pathway. PMID: 16467380
    14. Inp2p is the first peroxisomal protein implicated in the vectorial movement of peroxisomes.Inp2p interacts directly with the globular tail of Myo2p. PMID: 16678774
    15. Considering that a poly-IQ sequence is the lever arm of myosin V, data suggest that the arm may harbor a joint, localized within the IQ4 sequence, enabling the elasticity of the neck of myosin V. PMID: 18020453
    16. P-body disassembly is delayed in the motor mutant, myo2-66, indicating that Myo2p may facilitate the release of mRNAs from the P-body. PMID: 18218704
    17. Myo2 plays an important and direct role for mitochondrial motility and inheritance in budding yeast PMID: 18391073
    18. Ypt11 and Ret2 are components of a Myo2 receptor complex that functions during the Golgi inheritance into the growing bud. PMID: 18595704
    19. Fus2p and Sec4p, another Myo2p cargo associated with exocytotic vesicles, reside predominantly on different cellular organelles. PMID: 19403698
    20. Results delineate the region of Myo2p devoted to binding peroxisomes. PMID: 19687257

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  • 亞細胞定位：
    Bud neck. Bud tip. Note=Concentrates to sites of polarized growth, namely to the bud tip during S and G2 phases of the cell cycle and to the bud neck during cytokinesis.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
  • 數據庫鏈接：

    KEGG: sce:YOR326W

    STRING: 4932.YOR326W



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