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  4. Recombinant Saccharomyces cerevisiae Monothiol glutaredoxin-3 (GRX3)

Recombinant Saccharomyces cerevisiae Monothiol glutaredoxin-3 (GRX3)

  • 中文名稱：
    釀酒酵母GRX3重組蛋白
  • 貨號(hào)：
    CSB-YP312741SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母GRX3重組蛋白
  • 貨號(hào)：
    CSB-EP312741SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母GRX3重組蛋白
  • 貨號(hào)：
    CSB-EP312741SVG-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母GRX3重組蛋白
  • 貨號(hào)：
    CSB-BP312741SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母GRX3重組蛋白
  • 貨號(hào)：
    CSB-MP312741SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    GRX3
  • Uniprot No.：
  • 別名：
    GRX3; YDR098C; YD8557.05CMonothiol glutaredoxin-3
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-285
  • 氨基酸序列
    MCSFQVPSAF SFNYTSYCYK RHQARYYTAA KLFQEMPVIE INDQEQFTYL TTTAAGDKLI VLYFHTSWAE PCKALKQVFE AISNEPSNSN VSFLSIDADE NSEISELFEI SAVPYFIIIH KGTILKELSG ADPKEYVSLL EDCKNSVNSG SSQTHTMENA NVNEGSHNDE DDDDEEEEEE TEEQINARLT KLVNAAPVML FMKGSPSEPK CGFSRQLVGI LREHQVRFGF FDILRDESVR QNLKKFSEWP TFPQLYINGE FQGGLDIIKE SLEEDPDFLQ HALQS
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters. Binds one iron-sulfur cluster per dimer. The iron-sulfur cluster is bound between subunits, and is complexed by a bound glutathione and a cysteine residue from each subunit (Probable).
  • 基因功能參考文獻(xiàn)：
    1. Slt2 form iron/sulphur bridged clusters with Grx3 and Grx4. PMID: 28007574
    2. This article reports mechanistic studies that investigate the role of exogenous glutathione in defining cluster chirality, ligand exchange, and the cluster transfer chemistry of Saccharomyces cerevisiae Grx3. PMID: 28836015
    3. RNR cofactor biogenesis requires the ISC machinery to mature the Grx3/4 and Dre2 Fe-S proteins, which then function in iron and electron delivery to RNR, respectively. PMID: 28515324
    4. diferric tyrosyl radical formation in Saccharomyces cerevisiae Rnr2 protein: requirement of Rnr4 and contribution of Grx3/4 AND Dre2 proteins.( PMID: 21931161
    5. The authors present data showing that both Grx3 and Grx4 have roles in actin cytoskeleton remodeling and in cellular defenses against oxidative stress caused by reactive oxygen species (ROS) accumulation. PMID: 20889785
    6. Grx3 and Grx4 are novel components required for Aft1 iron regulation that most likely occurs in the nucleus PMID: 16648636
    7. crystal structure of the thioredoxin-like domain determined at 1.5 A resolution. loop containing signature motif WAxxC is partially disordered, indicating greater flexibility compared with classical dithiol thioredoxins with a WCGPC active-site motif. PMID: 18703840
    8. The ability of the Fra2-Grx3/4 complex to assemble a [2Fe-2S] cluster may act as a signal to control the iron regulon in response to cellular iron status in yeast. PMID: 19715344

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  • 蛋白家族：
    Glutaredoxin family, Monothiol subfamily
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: sce:YDR098C

    STRING: 4932.YDR098C



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