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  4. Recombinant Saccharomyces cerevisiae Mitochondrial inner membrane i-AAA protease supercomplex subunit YME1 (YME1), partial

Recombinant Saccharomyces cerevisiae Mitochondrial inner membrane i-AAA protease supercomplex subunit YME1 (YME1), partial

  • 中文名稱：
    釀酒酵母YME1重組蛋白
  • 貨號：
    CSB-YP335768SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母YME1重組蛋白
  • 貨號：
    CSB-EP335768SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母YME1重組蛋白
  • 貨號：
    CSB-EP335768SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母YME1重組蛋白
  • 貨號：
    CSB-BP335768SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母YME1重組蛋白
  • 貨號：
    CSB-MP335768SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    YME1
  • Uniprot No.：
  • 別名：
    YME1; OSD1; YTA11; YPR024W; YP9367.04; Mitochondrial inner membrane i-AAA protease supercomplex subunit YME1; EC 3.4.24.-; Protein OSD1; Tat-binding homolog 11; Yeast mitochondrial escape protein 1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalytic subunit of the mitochondrial inner membrane i-AAA protease supercomplex required for mitochondrial inner membrane protein turnover. The protease is probably ATP-dependent. Important to maintain the integrity of the mitochondrial compartment. Required both for the degradation of unassembled subunit 2 of cytochrome c oxidase (COX2) and for efficient assembly of mitochondrial respiratory chain. Binds unfolded substrates in an ATPase-independent manner; binding of folded COX2, a physiological substrate, requires an active ATPase but when COX2 is destabilized an active ATPase is no longer necessary.
  • 基因功能參考文獻：
    1. this study presents an atomic model of a substrate-bound inner mitochondrial membrane AAA+ quality control protease in yeast, YME1. PMID: 29097521
    2. Yme1 role in Tim10 degradation PMID: 26182355
    3. identify an important role for the mitochondrial protease Yme1 in the ability of cells that lack tafazzin function to maintain mitochondrial structural integrity and mitochondrial quality control and to undergo mitophagy PMID: 25688091
    4. No individual cysteine residue is required for the function of Tim9 or Tim10 in yeast and defective assembly of the small TIMs induces their proteolytic clearance from mitochondria via the mitochondrial ATP-dependent protease, Yme1. PMID: 23036860
    5. Inactivation of either Yme1 or Yta10/12, allows fermentative growth of hsc82 or hsp82 strains at 37 degrees C. PMID: 21439406
    6. Yme1 has a new function in protein translocation, indicating that the intermembrane space harbors diverse pathways for protein translocation. PMID: 16966379
    7. There are two distinct substrate binding sites in the i-AAA protease subunit Yme1. PMID: 17261594
    8. The phosphatidylethanolamine level of yeast mitochondria is affected by the mitochondrial components OXA1 and YME1. PMID: 17976194

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  • 亞細胞定位：
    Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. Note=Although this protein does not have any predicted transmembrane helices it behaves like an integral membrane protein.
  • 蛋白家族：
    AAA ATPase family; Peptidase M41 family
  • 數據庫鏈接：

    KEGG: sce:YPR024W

    STRING: 4932.YPR024W



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