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  4. Recombinant Saccharomyces cerevisiae Midasin (MDN1), partial

Recombinant Saccharomyces cerevisiae Midasin (MDN1), partial

  • 中文名稱：
    釀酒酵母MDN1重組蛋白
  • 貨號：
    CSB-YP607696SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母MDN1重組蛋白
  • 貨號：
    CSB-EP607696SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母MDN1重組蛋白
  • 貨號：
    CSB-EP607696SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母MDN1重組蛋白
  • 貨號：
    CSB-BP607696SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母MDN1重組蛋白
  • 貨號：
    CSB-MP607696SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MDN1
  • Uniprot No.：
  • 別名：
    MDN1; REA1; YLR106C; L2901; L8004.13Midasin; Dynein-related AAA-ATPase REA1; MIDAS-containing protein; Ribosome export/assembly protein 1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Nuclear chaperone required for maturation and nuclear export of pre-60S ribosome subunits. Functions at successive maturation steps to remove ribosomal factors at critical transition points, first driving the exit of early pre-60S particles from the nucleolus and then driving late pre-60S particles from the nucleus. At an early stage in 60S maturation, mediates the dissociation of the NOP7 complex (YTM1-ERB1-NOP7) from early pre-60S particles, rendering them competent for export from the nucleolus to the nucleoplasm. Subsequently recruited to the nucleoplasmic particles through interaction with the RIX1 complex. This binding is only possible if the 5S RNP at the central protuberance has undergone the rotation to complete its maturation. After remodeling, removes the ribosome biogenesis factor RSA4 in an ATP hydrolysis-driven step from pre-60S ribosomal subunits, rendering them competent for export from the nucleoplasm to the cytoplasm. Activates the GTPase activity of NOG2, which disengages from the pre-60S particle upon GTP hydrolysis, thus freeing its binding site for the nuclear export factor NMD3.
  • 基因功能參考文獻：
    1. Release of Nug2 from pre-60S particles requires both its K(+)-dependent GTPase activity and the remodelling ATPase activity of Rea1. PMID: 24240281
    2. Data suggest that Rea1 functions at successive maturation steps to remove ribosomal factors at critical transition points, first driving the exit of early pre-60S particles from the nucleolus and then driving late pre-60S particles from the nucleus. PMID: 20542003
    3. Rea1 is a dynein-related nuclear AAA-ATPase is involved in late rRNA processing and nuclear export of 60 S subunits PMID: 15528184
    4. Rea1 aligns with its interacting partners on the preribosome to effect a necessary step on the path to the export-competent 60S subunit. PMID: 19737519
  • 亞細胞定位：
    Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm.
  • 蛋白家族：
    Midasin family
  • 數據庫鏈接：

    KEGG: sce:YLR106C

    STRING: 4932.YLR106C



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