Recombinant Saccharomyces cerevisiae Intron-encoded endonuclease I-SceI (SCEI)

中文名稱(chēng)：

釀酒酵母SCEI重組蛋白
貨號(hào)：

CSB-YP361175SVG
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱(chēng)：

釀酒酵母SCEI重組蛋白
貨號(hào)：

CSB-EP361175SVG
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱(chēng)：

釀酒酵母SCEI重組蛋白
貨號(hào)：

CSB-EP361175SVG-B
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱(chēng)：

釀酒酵母SCEI重組蛋白
貨號(hào)：

CSB-BP361175SVG
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱(chēng)：

釀酒酵母SCEI重組蛋白
貨號(hào)：

CSB-MP361175SVG
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

SCEI
Uniprot No.：

P03882
別名：

SCEI; OMEGA; SECY; Q0160; Intron-encoded endonuclease I-SceI; EC 3.1.-.-; 21S rRNA intron maturase; Homing endonuclease omega
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-235
氨基酸序列

MKNIKKNQVM NLGPNSKLLK EYKSQLIELN IEQFEAGIGL ILGDAYIRSR DEGKTYCMQF EWKNKAYMDH VCLLYDQWVL SPPHKKERVN HLGNLVITWG AQTFKHQAFN KLANLFIVNN KKTIPNNLVE NYLTPMSLAY WFMDDGGKWD YNKNSTNKSI VLNTQSFTFE EVEYLVKGLR NKFQLNCYVK INKNKPIIYI DSMSYLIFYN LIKPYLIPQM MYKLPNTISS ETFLK
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Mitochondrial DNA endonuclease involved in intron homing. It introduces a specific double-strand break in the DNA of the 21S rRNA gene and thus mediates the insertion of an intron, containing its own coding sequence (group I intron), into an intronless gene. Specifically recognizes and cleaves the sequence 5'-TAGGGATAACAGGGTAAT-3'.
基因功能參考文獻(xiàn)：
1. findings suggest that the HSP70-subunit shields Endo.SceI from heat inactivation through ATP/ADP binding PMID: 15618627
2. A metal binding site is present in the active site of I-SceI that cleaves the top strand DNA. PMID: 18424798
亞細(xì)胞定位：

Mitochondrion.
蛋白家族：

LAGLIDADG endonuclease family
數(shù)據(jù)庫(kù)鏈接：

KEGG: sce:Q0160

STRING: 4932.Q0160