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  4. Recombinant Saccharomyces cerevisiae Inositol phosphosphingolipids phospholipase C (ISC1), partial

Recombinant Saccharomyces cerevisiae Inositol phosphosphingolipids phospholipase C (ISC1), partial

  • 中文名稱：
    釀酒酵母ISC1重組蛋白
  • 貨號：
    CSB-YP340193SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母ISC1重組蛋白
  • 貨號：
    CSB-EP340193SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母ISC1重組蛋白
  • 貨號：
    CSB-EP340193SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母ISC1重組蛋白
  • 貨號：
    CSB-BP340193SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母ISC1重組蛋白
  • 貨號：
    CSB-MP340193SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ISC1
  • Uniprot No.：
  • 別名：
    ISC1; YER019W; Inositol phosphosphingolipids phospholipase C; IPS phospholipase C; IPS-PLC; EC 3.1.4.-; Neutral sphingomyelinase; N-SMase; nSMase
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Responsible for the hydrolysis of the phosphosphingolipids (IPS), inositol phosphorylceramide (IPC), mannosylinositol phosphorylceramide (MIPC), and mannosyldiinositol phosphorylceramide (M(IP)2C). Regulates sphingolipid metabolism in mitochondria, especially the formation of alpha-hydroxylated very long chain phytoceramides. The generated ceramides contribute to the normal function of mitochondria. Also active on sphingomyelin (SM), but this activity is probably not physiologically relevant.
  • 基因功能參考文獻：
    1. activation of Isc1p in vivo is dependent on PGS1 and on the mitochondrial phospholipids PG/CL PMID: 15611094
    2. results showed that Isc1p contributes specifically to the formation of the C24-, C24:1- and C26-dihydroceramide species PMID: 16201964
    3. Isc1 regulates sphingolipid metabolism in yeast mitochondria. PMID: 17880915
    4. These results indicate that Isc1p plays an important role in the regulation of cellular redox homeostasis, through modulation of iron levels, and of apoptosis. PMID: 18162582
    5. Findings related to the function of ISC1, its localization, mechanisms, and its roles in cell response to different types of stresses. [REVIEW] PMID: 18305313
    6. These results suggest a novel role for Isc1p in allowing the reprogramming of gene expression during the transition from anaerobic to aerobic metabolism. PMID: 19179331

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  • 亞細胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion outer membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Neutral sphingomyelinase family
  • 數據庫鏈接：

    KEGG: sce:YER019W

    STRING: 4932.YER019W



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