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  4. Recombinant Saccharomyces cerevisiae Inner centromere protein-related protein SLI15 (SLI15), partial

Recombinant Saccharomyces cerevisiae Inner centromere protein-related protein SLI15 (SLI15), partial

  • 中文名稱：
    釀酒酵母SLI15重組蛋白
  • 貨號：
    CSB-YP339944SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母SLI15重組蛋白
  • 貨號：
    CSB-EP339944SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母SLI15重組蛋白
  • 貨號：
    CSB-EP339944SVG-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母SLI15重組蛋白
  • 貨號：
    CSB-BP339944SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母SLI15重組蛋白
  • 貨號：
    CSB-MP339944SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SLI15
  • Uniprot No.：
  • 別名：
    SLI15; YBR156C; YBR1206; Inner centromere protein-related protein SLI15; INCENP-related protein SLI15
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Required for high-fidelity chromosome segregation during the later part of each cell cycle in association with the protein kinase IPL1. Stimulates IPL1 kinase activity and facilitates its association with the mitotic spindle. Has a role in attaching the kinetochores to the microtubules and ensuring that sister kinetochores connect to opposite poles.
  • 基因功能參考文獻(xiàn)：
    1. Ipl1-dependent phosphorylation of Sli15 therefore inhibits its association with microtubules both in vivo and in vitro and may negatively regulate the tension checkpoint mechanism. PMID: 24558497
    2. phosphorylation of INCENP/Sli15 is required for activation of the kinase Aurora B/Ipl1 and can occur in trans. PMID: 23814063
    3. an engineered truncation of the Sli15 subunit of budding yeast chromosomal passenger complex that eliminates association with the inner centromere nevertheless supports proper chromosome segregation during both mitosis and meiosis PMID: 23604256
    4. Cdk1 and Ipl1/Aurora cooperatively modulate microtubule dynamics and that Ipl1/Aurora-dependent phosphorylation of Sli15 controls spindle function by excluding the CPC from spindle regions engaged in microtubule polymerization PMID: 21727193
    5. dephosphorylation by Cdc14 inactivates the mitotic checkpoint PMID: 20619650
    6. Using an in vitro approach based on the sequence-specific budding yeast centromere, a complex of the chromosomal passenger proteins Bir1 and Sli15 (Survivin and INCENP) that links centromeres to microtubules was identified. PMID: 17174893
    7. The interaction between Bir1p and Sli15p is specifically involved in regulating septin dynamics during anaphase. PMID: 17652458
    8. Findings reveal a new role for the central kinetochore in promoting Sli15 function, and suggest functional Ame1 is required for the correction of defective attachments by promoting the localization of Sli15/INCENP at kinetochores. PMID: 19597337

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  • 亞細(xì)胞定位：
    Nucleus. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore.
  • 蛋白家族：
    INCENP family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YBR156C

    STRING: 4932.YBR156C



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