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  4. Recombinant Saccharomyces cerevisiae Increased sodium tolerance protein 2 (IST2), partial

Recombinant Saccharomyces cerevisiae Increased sodium tolerance protein 2 (IST2), partial

  • 中文名稱：
    釀酒酵母IST2重組蛋白
  • 貨號：
    CSB-YP327804SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母IST2重組蛋白
  • 貨號：
    CSB-EP327804SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母IST2重組蛋白
  • 貨號：
    CSB-EP327804SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母IST2重組蛋白
  • 貨號：
    CSB-BP327804SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母IST2重組蛋白
  • 貨號：
    CSB-MP327804SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    IST2
  • Uniprot No.：
  • 別名：
    IST2; YBR086C; YBR0809; Increased sodium tolerance protein 2
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    May be involved in ion homeostasis together with BTN1 or BTN2.
  • 基因功能參考文獻：
    1. The authors show that, in BY4741 background, the lack of Ist2 results in the accumulation of higher amounts of sodium when the cells are exposed to the presence of this cation, demonstrating the importance of Ist2 for the maintenance of low intracellular levels of toxic sodium. PMID: 28199631
    2. IST2 localization depends on the presence of a PM-binding domain, an intrinsically disordered linker region of sufficient length. PMID: 25409870
    3. Data suggest that Btn2p, along with Btn1 and Ist2, may play a regulatory role across the cell in response to alterations in the intracellular environment. PMID: 15701790
    4. Based on these observations, we suggest a local synthesis of Ist2p at cortical ER sites, from where the protein is sorted by a novel mechanism to the plasma membrane. PMID: 15911878
    5. posttranslational mechanism, which leads to the concentration of Ist2--via multimerization--at ER sites, followed by direct transport to the plasma membrane PMID: 17234190
    6. Results suggest that a direct interaction of the Ist2 sorting signal with lipids at the plasma membrane places Ist2 at contact sites between cortical ER and plasma membrane. PMID: 19208765
    7. Data show that binding of phosphatidylinositol phosphates leads to efficient accumulation of Ist2 at domains of the cortical ER from where the protein may reach the PM independently of the function of the sec-pathway. PMID: 19453974

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein. Note=Correct localization requires BTN2. Localizes to the mother cell in small budded cells and to the bud in medium and large budded cells. Transported to the bud tip by an actomyosin based process. Compartmentalization maintained by a septin mediated membrane diffusion barrier at the mother-bud neck.
  • 數據庫鏈接：

    KEGG: sce:YBR086C

    STRING: 4932.YBR086C



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