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  4. Recombinant Saccharomyces cerevisiae Histone H2B.1 (HTB1)

Recombinant Saccharomyces cerevisiae Histone H2B.1 (HTB1)

  • 中文名稱：
    釀酒酵母HTB1重組蛋白
  • 貨號：
    CSB-YP355824SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母HTB1重組蛋白
  • 貨號：
    CSB-EP355824SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母HTB1重組蛋白
  • 貨號：
    CSB-EP355824SVG-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母HTB1重組蛋白
  • 貨號：
    CSB-BP355824SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母HTB1重組蛋白
  • 貨號：
    CSB-MP355824SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    HTB1
  • Uniprot No.：
  • 別名：
    H2B1_YEAST; Histone H2B.1; Histone H2B.2; HTB 1; HTB 2; HTB1; Htb1p; HTB2; Htb2p; SPT 12; SPT12; Suppressor of Ty protein 12
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    2-131
  • 氨基酸序列
    SAKAEKKPA SKAPAEKKPA AKKTSTSTDG KKRSKARKET YSSYIYKVLK QTHPDTGISQ KSMSILNSFV NDIFERIATE ASKLAAYNKK STISAREIQT AVRLILPGEL AKHAVSEGTR AVTKYSSSTQ A
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 基因功能參考文獻(xiàn)：
    1. This study not only demonstrates the applications of the versatile histone library, but also reveals many previously unknown functions of histone H2A and H2B. PMID: 29038170
    2. Data indicate that histone H2B ubiquitylation (H2Bub) and Asf1 cooperatively promote transcriptional silencing at telomeres and mating loci. PMID: 28520954
    3. A Nap1 dimer provides an acidic binding surface and asymmetrically engages a single H2A-H2B heterodimer. PMID: 27225933
    4. Chd1 takes part in chromatin reorganization surrounding transcribing RNA polymerase II (Pol II) in asymmetric nucleosomes; ubiquitin-conjugated histone H2B stimulates nucleosome sliding by Chd1. PMID: 28032848
    5. decreased CKII function in vivo results in decreased levels of histone H2B lysine 123 monoubiquitylation, a modification dependent on FACT and PAF-C. PMID: 27143358
    6. Nap1 is essential for maintaining proper chromatin composition and modulating the exchange of H2A-H2B in vivo. PMID: 26884462
    7. A role for the H2B nucleosome acidic patch in recruitment of histone modification machinery and maintenance of transcriptional integrity. PMID: 26241481
    8. FACT, a heterodimer of Spt16 and Pob3, disrupts nucleosome structure by binding H2A-H2B with conserved peptide motifs. PMID: 26455391
    9. A histone modification domain (HMD), when expressed as the only source of Rtf1, can promote H3 K4 and K79 methylation and H2B K123 ubiquitylation in yeast. PMID: 22699496
    10. lysine 123 affects transcription in yeast primarily because of it being a site of ubiquitylation in HTB1 PMID: 17447102
    11. A continued accumulation of the histone mRNA HTB1 appears to result from a delay in exit from S-phase in rrp6 deleted cells. PMID: 17855393
    12. analysis of the NMR structure of budding yeast chaperone Chz1 complexed with histones H2A.Z-H2B PMID: 18641662
    13. A yeast strain unable to be ubiquitylated on H2B (K123R) is still proficient for H3 trimethylation at both K4 and K79, indicating that H3 methylation status is not solely dependent on H2B ubiquitylation. PMID: 19255247

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  • 亞細(xì)胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H2B family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YDR224C

    STRING: 4932.YDR224C



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