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  4. Recombinant Saccharomyces cerevisiae Guanine nucleotide exchange factor LTE1 (LTE1), partial

Recombinant Saccharomyces cerevisiae Guanine nucleotide exchange factor LTE1 (LTE1), partial

  • 中文名稱：
    釀酒酵母LTE1重組蛋白
  • 貨號(hào)：
    CSB-YP362252SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母LTE1重組蛋白
  • 貨號(hào)：
    CSB-EP362252SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母LTE1重組蛋白
  • 貨號(hào)：
    CSB-EP362252SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母LTE1重組蛋白
  • 貨號(hào)：
    CSB-BP362252SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母LTE1重組蛋白
  • 貨號(hào)：
    CSB-MP362252SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    LTE1
  • Uniprot No.：
  • 別名：
    LTE1; EIS4; MSI2; YAL024CGuanine nucleotide exchange factor LTE1; Low temperature essential protein 1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    GDP-GTP exchange factor for TEM1, a Ras-like protein, component of the mitotic exit network (MEN). Activation of TEM1 by LTE1 in the bud ultimately leads to activation of CDC15 followed by the release of CDC14 from the nucleolus, which then inactivates cyclin-dependent kinases (CDKs) activity by several mechanism. Required for TEM1 localization to the bud cortex during mitotic exit. Fine-tunes the timing of the mitotic exit and couples this event with cytokinesis.; Involved in proprotein-processing like proalpha factor-processing in the secretory pathway.
  • 基因功能參考文獻(xiàn)：
    1. Data show that bud-localized MEN regulator Lte1 prevents Kin4 that escapes into the bud from associating with SPBs in the daughter cell. PMID: 21709215
    2. Data suggest that Lte1 promotes mitotic exit by inhibiting Kin4 activity at the daughter spindle pole body. PMID: 21670215
    3. Phosphorylation of Lte1 by Cdk prevents polarized growth during mitotic arrest in S. cerevisiae PMID: 21149565
    4. Our findings suggest that Cla4-dependent phosphorylation promotes the initial association of Lte1 with Ras at the bud cortex and that Ras is required to stabilize phosphorylated forms of Lte1 at the bud cortex. PMID: 15917658
    5. analysis of a link between the mitotic regulator, Lte1p, and protein processing and trafficking in the secretory/endosomal system PMID: 17121813
    6. In lteDelta cells Brn1 release was delayed, even at temperatures that do not impair mitotic exit. PMID: 19387493
    7. Lte1 can determine the subcellular localization of Bfa1 at spindle pole bodies (SPB) by promoting the loss of Bfa1 from the maternal SPB. PMID: 19948498

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  • 亞細(xì)胞定位：
    Cytoplasm. Bud. Note=The localization to the bud requires interaction with KEL1, as well as the presence of septins, CDC42, CLA4 and RAS2.
  • 蛋白家族：
    LTE1 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YAL024C

    STRING: 4932.YAL024C



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