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  4. Recombinant Saccharomyces cerevisiae Fork head protein homolog 1 (FKH1)

Recombinant Saccharomyces cerevisiae Fork head protein homolog 1 (FKH1)

  • 中文名稱：
    釀酒酵母FKH1重組蛋白
  • 貨號：
    CSB-YP340254SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母FKH1重組蛋白
  • 貨號：
    CSB-EP340254SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母FKH1重組蛋白
  • 貨號：
    CSB-EP340254SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母FKH1重組蛋白
  • 貨號：
    CSB-BP340254SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母FKH1重組蛋白
  • 貨號：
    CSB-MP340254SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    FKH1
  • Uniprot No.：
  • 別名：
    FKH1; YIL131C; Fork head protein homolog 1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-484
  • 氨基酸序列
    MSVTSREQKF SGKYSSYTAQ DRQGLVNAVT CVLSSSSDPV AVSSDYSNSL SIAREVNAYA KIAGCDWTYY VQKLEVTIGR NTDSLNLNAV PGTVVKKNID IDLGPAKIVS RKHAAIRFNL ESGSWELQIF GRNGAKVNFR RIPTGPDSPP TVLQSGCIID IGGVQMIFIL PEQETIISDY CLNHLMPKLL STYGTNGNNN PLLRNIIEGS TYLREQRLQE EARLQQLDHL HTPLSSSSDV NPIGDPHGDT IMMEEDEEDE NYTRGGIRPN TYTSSSNNAV TNGNVPHIEN PSDLSLDENR YIKPPQSYAS MITQAILSTP EGSISLADIY KFISDNYAFY RFSQMAWQNS VRHNLSLNKA FEKVPKRAGQ QGKGMNWKIS DEVRRDFLNK WNAGKLSKIR RGASVTRQLQ LHMSKFGEIP APESSSIDPR GIKAQKVKKS LQATSSILGE SAPQLQRTQL TGQISTTTSM DVTTNANVNN SSLS
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Transcription factor that regulates the expression of the CLB2 cluster of genes during the G2/M phase of the mitotic cell cycle. The CLB2 cluster of genes includes mitotic regulators such as CLB1, CLB2, CDC5 and CDC20 as well as SWI5 and ACE2, transcription factors required for the subsequent temporal wave of cell cycle regulated gene expression in the M/G1 phase interval. Involved in HMRa silencing. FKH1 and FKH2 associate with the coding regions of active genes and influence, in opposing ways, transcriptional elongation and termination, and coordinate early transcription elongation and pre-mRNA processing. Both FKH1 and FKH2 play a role as regulators of lifespan in collaboration with the anaphase-promoting complex (APC), likely through combined regulation of stress response, genomic stability, and cell cycle regulation. FKH1 and FKH2 function also in controlling yeast cell morphology by preventing preudohyphal growth. Acts as a rate-limiting replication origin activator via its interactin with the origin recognition complex (ORC). Plays a transcription-independent role in recombination donor preference during mating-type switching through binding to the recombination enhancer (RE), a 700-bp cis-acting element that controls recombination along the left arm of chromosome III.
  • 基因功能參考文獻：
    1. Fkh1 and Fkh2 dimerization controls DNA replication timing and spatial organization of chromosomes. PMID: 28265091
    2. Spatial and directional arrangement of Fkh1/2 sites is crucial for efficient binding of the Fkh1 protein and for early firing of the origins. PMID: 28141805
    3. We have shown that the phosphothreonine binding motif of the FHA domain of Fkh1 plays a critical role in the regulation of donor preference during Saccharomyces cerevisiae mating-type switching PMID: 22496671
    4. This study establishes the FKH1 and FKH2 role as evolutionarily conserved regulators of lifespan in yeast and identifies the APC as a novel component of this mechanism PMID: 22438832
    5. Study shows that Fkh1 and Fkh2 regulate the initiation timing of most of the earliest origins in the yeast genome through a novel mechanism involving origin clustering in G1 phase. PMID: 22265405
    6. Disruption of fkh1-associated cell cycle transcription by oxidative stress PMID: 15371544
    7. Fkh1 binds to the recombination enhancer only in G(2), which contrasts with its cell cycle-independent binding to its other target promoters. PMID: 16809780
    8. These and other data supported a model in which FKH1 and CLB5 modulated Sir2-4 chromatin and late-origin firing through opposing regulation of a common pathway. PMID: 18045995

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  • 亞細胞定位：
    Nucleus. Cytoplasm, cytosol.
  • 數據庫鏈接：

    KEGG: sce:YIL131C

    STRING: 4932.YIL131C



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