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  4. Recombinant Saccharomyces cerevisiae Eukaryotic translation initiation factor 1A (TIF11)

Recombinant Saccharomyces cerevisiae Eukaryotic translation initiation factor 1A (TIF11)

  • 中文名稱：
    釀酒酵母TIF11重組蛋白
  • 貨號：
    CSB-YP007507SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母TIF11重組蛋白
  • 貨號：
    CSB-EP007507SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母TIF11重組蛋白
  • 貨號：
    CSB-EP007507SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母TIF11重組蛋白
  • 貨號：
    CSB-BP007507SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母TIF11重組蛋白
  • 貨號：
    CSB-MP007507SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    TIF11
  • Uniprot No.：
  • 別名：
    TIF11; YMR260C; YM8156.02CEukaryotic translation initiation factor 1A; eIF-1A; Eukaryotic translation initiation factor 4C; eIF-4C
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-153
  • 氨基酸序列
    MGKKNTKGGK KGRRGKNDSD GPKRELIYKE EGQEYAQITK MLGNGRVEAS CFDGNKRMAH IRGKLRKKVW MGQGDIILVS LRDFQDDQCD VVHKYNLDEA RTLKNQGELP ENAKINETDN FGFESDEDVN FEFGNADEDD EEGEDEELDI DDI
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.
  • 基因功能參考文獻：
    1. eIF5-CTD (amino acids 241-405; Tif5p-B6) interacted with eIF1A (Tif11p). PMID: 24319994
    2. eIF1 autoregulates translation in yeast and discrimination against poor sequence context in vivo depends on specific domains and residues in eIF1, eIF1A, and eIF2beta PMID: 21930786
    3. The OB-fold is crucial for ribosome-binding and the C-terminal domain of eIF1A has eukaryotic-specific functions in ternary complex recruitment and scanning. PMID: 16193068
    4. Data suggest strongly that the interaction between eIF1A and eIF5 is involved in maintaining the fidelity of start codon AUG recognition in vivo. PMID: 16380131
    5. Location of the mutations suggests that their mechanism of suppression may depend on effects on the conserved G-protein elements: the P-loop and nucleotide-binding element. PMID: 16951075
    6. N- and C-terminal residues of eIF1A have opposing effects on the fidelity of start codon selection. PMID: 17332751
    7. Our results suggest that eIF1 and eIF1A promote an open, scanning-competent preinitiation complex that closes upon start codon recognition and eIF1 release to stabilize ternary complex binding and clamp down on mRNA. PMID: 17434125
    8. A pair of repeats in the eIF1A CTT, dubbed Scanning Enhancer 1 (SE1) and SE2, was found to stimulate recruitment of Met-tRNA(i)(Met) in the ternary complex (TC) with eIF2.GTP and also to block initiation at UUG codons PMID: 20048003

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  • 蛋白家族：
    EIF-1A family
  • 數據庫鏈接：

    KEGG: sce:YMR260C

    STRING: 4932.YMR260C



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