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Recombinant Saccharomyces cerevisiae Dynamin-related protein DNM1 (DNM1), partial

  • 中文名稱:
    釀酒酵母DNM1重組蛋白
  • 貨號:
    CSB-YP345810SVG
  • 規(guī)格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    釀酒酵母DNM1重組蛋白
  • 貨號:
    CSB-EP345810SVG
  • 規(guī)格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    釀酒酵母DNM1重組蛋白
  • 貨號:
    CSB-EP345810SVG-B
  • 規(guī)格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    釀酒酵母DNM1重組蛋白
  • 貨號:
    CSB-BP345810SVG
  • 規(guī)格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    釀酒酵母DNM1重組蛋白
  • 貨號:
    CSB-MP345810SVG
  • 規(guī)格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
  • Uniprot No.:
  • 別名:
    DNM1; YLL001W; L1381Dynamin-related protein DNM1; EC 3.6.5.5
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Microtubule-associated force-producing protein that participates mitochondrial fission. Fission of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. Functions antagonistically with FZO1.
  • 基因功能參考文獻:
    1. A double deletion mutant in which two essential components of the fission and fusion machinery, Dnm1 and Mgm1, are simultaneously ablated are characterized by impaired respiration and a decrease in mitophagy and replicative lifespan PMID: 25601284
    2. the Insert B domain of the Dnm1 guanosine triphosphatase (a DRP) contains a novel motif required for association with the mitochondrial adaptor Mdv1 PMID: 23148233
    3. The de novo synthesis of peroxisomes is independent of the GTPases Vps1p and Dnm1p, two proteins required for peroxisome fission. PMID: 20655617
    4. Molecular architecture of a dynamin adaptor: implications for assembly of mitochondrial fission complexes. PMID: 21149566
    5. Recruitment of Dnm1p to mitochondria is disrupted in mdv1delta caf4delta yeast, demonstrating that Mdv1p and Caf4p are molecular adaptors that recruit Dnm1p to mitochondrial fission sites PMID: 16009724
    6. dynamic interactions between Mdv1 and Dnm1 play a key role in division by regulating Dnm1 self-assembly PMID: 16272155
    7. Dnm1p dimers are initially recruited to the membrane via interaction with Mdv1p.Fis1p complexes PMID: 16601120
    8. Data suggest that Caf4p and Fis1, but not Mdv1, play a role in determining the polarized localization of those Dnm1p clusters that are not immediately involved in the mitochondrial fission process. PMID: 16835275
    9. Vps1p and Dnm1p control peroxisome abundance in Saccharomyces cerevisiae. PMID: 16968746
    10. We demonstrate that the dynamin-related protein 1 (Dnm1p), a mitochondrial fission protein conserved from yeast to humans, affects ageing in the two model systems we studied, Podospora anserina and Saccharomyces cerevisiae. PMID: 17173038
    11. Thus, our studies have revealed an additional role for Dnm1p in mitochondrial transmission through its interaction with Num1p, thereby providing a link between mitochondrial division and inheritance. PMID: 17336903
    12. the concave surface of the Fis1 tetratricopeptide repeat-like domain is evolutionarily conserved to bind the dynamin-like GTPase Dnm1 and not Mdv1 as previously predicted PMID: 17884824
    13. Woronin bodies emerge after the formation of a HEX1 core crystal within peroxisomes followed by Vps1p- and Dnm1p-mediated fission PMID: 18435762
    14. findings show that Dnm1p-dependent peroxisome fission requires Fis1p, Caf4p and Mdv1p PMID: 18445678
    15. Mdv1 played a postmitochondrial targeting role in division by interacting & coassembling with the GTP-bound form of Dnm1; this regulated interaction nucleated & promoted self-assembly of Dnm1 into helical structures which drive membrane scission PMID: 19679814

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  • 亞細胞定位:
    Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasm, localizes to the cytoplasmic outer membrane of mitochondria.
  • 蛋白家族:
    TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family
  • 數(shù)據(jù)庫鏈接:

    KEGG: sce:YLL001W

    STRING: 4932.YLL001W



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