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  4. Recombinant Saccharomyces cerevisiae DNA repair protein RAD59 (RAD59)

Recombinant Saccharomyces cerevisiae DNA repair protein RAD59 (RAD59)

  • 中文名稱：
    釀酒酵母RAD59重組蛋白
  • 貨號：
    CSB-YP619572SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母RAD59重組蛋白
  • 貨號：
    CSB-EP619572SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母RAD59重組蛋白
  • 貨號：
    CSB-BP619572SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母RAD59重組蛋白
  • 貨號：
    CSB-MP619572SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RAD59
  • Uniprot No.：
  • 別名：
    RAD59; YDL059C; DNA repair protein RAD59
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-238
  • 氨基酸序列
    MTIQAKPSSS ISYDSTTYGT APGLDIKEFQ IIEDWNGRPA SAWSVQRIGL LQSKIERYTY NIYHNNKYGK HNLSKLIPGH ALIQFANETF GYDGWRMDVI DVEARECQPF TAVNNGENTN TSEVKYTVVA EAQVKVTLKD GTNTQCGGLG RITLSSRGEC YNRSKKEAVG DALKKALLSF EKIILDYETK ITNNYYVDGL YGSKKIKNEA NTNYNLLSAT NSKPTFIKLE DAKGTHIK
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Involved in the repair of double-strand breaks in DNA during vegetative growth via recombination and single-strand annealing. Anneals complementary single-stranded DNA.
  • 基因功能參考文獻：
    1. propose that Rad59 promotes fork progression when Okazaki fragment processing is compromised and counteracts PCNA-K107 mediated cell cycle arrest PMID: 23824283
    2. SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination PMID: 27130983
    3. We show that Rad59-facilitated Y' translocations on X-only telomeres delay the onset of senescence while preparing ground for type I survivor formation. PMID: 25375789
    4. Findings suggest a Rad52/Rad59-dependent, recombination pathway that could ultimately lead to the removal of faulty flaps in conjunction with cohesion establishment factors. PMID: 24711454
    5. These results suggest that Rad59 influences the rescue of replication lesions by multiple recombination factors. PMID: 24125552
    6. Data show that RSC physically interacts with the recombination protein Rad59 and functions in homologous recombination. PMID: 21807899
    7. at least one role of Rad59 in translocation formation by single-strand annealing is supporting the machinery required for cleavage of non-homologous tails PMID: 20012294
    8. examined the ssDNA annealing activity of Rad52 and Rad59 proteins and found significant differences in their biochemical properties. PMID: 16565518
    9. S. cerevisiae Rad59 protein and its eukaryotic counterparts function by binding to single-stranded DNA formed as intermediates of recombination rather than by binding to the unprocessed DNA double-strand break. PMID: 17040915
    10. Data from this study show that defects in the Rad51-Sgs1-dependent and Sgs1-dependent lesion-bypass pathways activate Rad59-Rad1- and Rad59-dependent pathways, respectively, resulting in uSCR. PMID: 17399687
    11. RAD59 was found to play a particularly significant role in recombination between the short substrates that was partially independent of that of Rad52 DNA Repair and Recombination Protein PMID: 18373960

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    RAD52 family
  • 數據庫鏈接：

    KEGG: sce:YDL059C

    STRING: 4932.YDL059C



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