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  4. Recombinant Saccharomyces cerevisiae DNA polymerase zeta catalytic subunit (REV3), partial

Recombinant Saccharomyces cerevisiae DNA polymerase zeta catalytic subunit (REV3), partial

  • 中文名稱：
    釀酒酵母REV3重組蛋白
  • 貨號：
    CSB-YP019577SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母REV3重組蛋白
  • 貨號：
    CSB-EP019577SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母REV3重組蛋白
  • 貨號：
    CSB-EP019577SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母REV3重組蛋白
  • 貨號：
    CSB-BP019577SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母REV3重組蛋白
  • 貨號：
    CSB-MP019577SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    REV3
  • Uniprot No.：
  • 別名：
    REV3; PSO1; YPL167C; P2535DNA polymerase zeta catalytic subunit; EC 2.7.7.7; Protein reversionless 3
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Nonessential DNA polymerase. Required for DNA damage induced mutagenesis. Involved in DNA repair, mitochondrial DNA repair and translesion synthesis. Translesion synthesis in S.cerevisiae may use a specialized DNA polymerase that is not required for other DNA replicative processes. Has a role in the bypass of abasic (AP) sites. Highly inefficient in incorporating nucleotides opposite the AP site, but efficiently extends from nucleotides, particularly an A, inserted opposite the lesion.
  • 基因功能參考文獻：
    1. This study also uncovered an important role for Rev3 and Rev7 (but not for Pol32) subunits of polymerase zeta in the survival of cells undergoing telomere losses and forming palindromes PMID: 27334270
    2. A novel mutation affecting the catalytic subunit Rev3 and the role of Rev3 in the Pol32 regulation PMID: 24819597
    3. Strains deficient in Pol zeta (Rev3) show little bypass of the T-T (6-4) photoadduct or the abasic sites. PMID: 15520252
    4. a specific subset of the Pol zeta-dependent mutational events requires oxidative metabolism PMID: 16290107
    5. Physical association of Rev1 with DNA polymerase zeta is mediated through the carboxyl terminus of Rev1 and the polymerase domain of Rev3. PMID: 17030609
    6. error-prone REV3 pathway determines spontaneous mutagenesis in Saccharomyces cerevisiae PMID: 17396018
    7. Results imply that about 30% of starvation-associated base substitutions and frameshifts arise by some unknown mechanism that does not involve Pol zeta or NHEJ. PMID: 19305999
    8. mismatch repair system promotes Polzeta-dependent mutagenesis by inhibiting an alternative, error-free pathway that depends on homologous recombination PMID: 19307574
    9. The L979F Pol zeta, and perhaps wild-type Pol zeta, which also generates clustered mutations at a lower but significant rate, performs short patches of processive, error-prone DNA synthesis. PMID: 19380376

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  • 亞細胞定位：
    Mitochondrion. Nucleus.
  • 蛋白家族：
    DNA polymerase type-B family
  • 數據庫鏈接：

    KEGG: sce:YPL167C

    STRING: 4932.YPL167C



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