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  4. Recombinant Saccharomyces cerevisiae DNA polymerase alpha catalytic subunit A (POL1), partial

Recombinant Saccharomyces cerevisiae DNA polymerase alpha catalytic subunit A (POL1), partial

  • 中文名稱：
    釀酒酵母POL1重組蛋白
  • 貨號：
    CSB-YP321104SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母POL1重組蛋白
  • 貨號：
    CSB-EP321104SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母POL1重組蛋白
  • 貨號：
    CSB-EP321104SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母POL1重組蛋白
  • 貨號：
    CSB-BP321104SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母POL1重組蛋白
  • 貨號：
    CSB-MP321104SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    POL1
  • Uniprot No.：
  • 別名：
    POL1; CDC17; YNL102W; N2181DNA polymerase alpha catalytic subunit A; EC 2.7.7.7; DNA polymerase I subunit A; DNA polymerase alpha:primase complex p180 subunit; DNA polymerase-primase complex p180 subunit; Pol alpha-primase complex p180 subunit
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Catalytic component of DNA polymerase alpha, which in complex with DNA primase (DNA polymerase alpha:primase) constitutes a replicative polymerase. POL1 has a role in promoting telomere replication during interaction with CDC13.
  • 基因功能參考文獻：
    1. Degradation of specific nuclear proteins occurs in the cytoplasm in Saccharomyces cerevisiae PMID: 24621829
    2. an N-terminal region of Polalpha (Pol1p) that is evolutionarily conserved among different species interacts with Spt16p-Pob3p and Ctf4p in vivo PMID: 15485923
    3. In the yeast Saccharomyces cerevisiae, reduced levels of the replicative alpha DNA polymerase result in greatly elevated frequencies of chromosome translocations and chromosome loss. PMID: 15766523
    4. Report the 12 A cryo-electron microscopic structure for the complete 14-subunit yeast Pol I, a homology model for the core enzyme, and the crystal structure of the subcomplex A14/43. PMID: 18160037
    5. Low levels of Pol1p significantly increase recombination in the rDNA array, both between homologues and between sister chromatids. PMID: 18584028

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    DNA polymerase type-B family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YNL102W

    STRING: 4932.YNL102W



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