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Recombinant Saccharomyces cerevisiae Cell division cycle protein CDT1 (TAH11), partial

  • 中文名稱:
    釀酒酵母TAH11重組蛋白
  • 貨號:
    CSB-YP342459SVG
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    釀酒酵母TAH11重組蛋白
  • 貨號:
    CSB-EP342459SVG
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    釀酒酵母TAH11重組蛋白
  • 貨號:
    CSB-EP342459SVG-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    釀酒酵母TAH11重組蛋白
  • 貨號:
    CSB-BP342459SVG
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    釀酒酵母TAH11重組蛋白
  • 貨號:
    CSB-MP342459SVG
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    TAH11
  • Uniprot No.:
  • 別名:
    TAH11; CDT1; SID2; YJR046W; J1641Cell division cycle protein CDT1; SIC1 indispensable protein 2; Topoisomerase-A hypersensitive protein 11
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    DNA replication licensing factor, required for pre-replication complex assembly. Faithful duplication of the genetic material requires 'once per cell cycle' DNA replication initiation and elongation. Central to this control is the tightly regulated formation of prereplicative complexes (preRCs) at future origins of DNA replication. Required for the recruitment of the MCM2-7 helicase complex to the replication origins.
  • 基因功能參考文獻:
    1. Cdt1 acts as a brace, holding MCM open for DNA entry and bound to ATP until ORC-Cdc6 triggers ATP hydrolysis by MCM, promoting both Cdt1 ejection and MCM ring closure in Saccharomyces cerevisiae PMID: 28643783
    2. theses data support a model in which origin-bound ORC and Cdc6 recruit two Cdt1 molecules to initiate double-hexamer formation prior to helicase loading and demonstrate that Cdt1 influences the replication competence of loaded Mcm2-7 helicases. PMID: 22045335
    3. Consistent with the inactivation of one Cdt1-binding site preventing helicase loading, CDK phosphorylation of ORC causes a twofold reduction of initial Cdt1/Mcm2-7 recruitment but results in nearly complete inhibition of Mcm2-7 loading. PMID: 21289063
  • 亞細胞定位:
    Cytoplasm. Nucleus. Note=Undergoes cell cycle-dependent changes in its nuclear localization. Exits the nucleus and remains in the cytoplasm during S phase through early mitosis, and re-accumulates in the nucleus around the end of mitosis.
  • 蛋白家族:
    Cdt1 family
  • 數據庫鏈接:

    KEGG: sce:YJR046W

    STRING: 4932.YJR046W



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