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  4. Recombinant Saccharomyces cerevisiae Autophagy-related protein 9 (ATG9), partial

Recombinant Saccharomyces cerevisiae Autophagy-related protein 9 (ATG9), partial

  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Autophagy-related protein 9(ATG9) ,partial
  • 貨號：
    CSB-YP615466SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Autophagy-related protein 9(ATG9) ,partial
  • 貨號：
    CSB-EP615466SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Autophagy-related protein 9(ATG9) ,partial
  • 貨號：
    CSB-EP615466SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Autophagy-related protein 9(ATG9) ,partial
  • 貨號：
    CSB-BP615466SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Autophagy-related protein 9(ATG9) ,partial
  • 貨號：
    CSB-MP615466SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ATG9
  • Uniprot No.：
  • 別名：
    ATG9; APG9; AUT9; CVT7; YDL149W; D1560Autophagy-related protein 9; Cytoplasm to vacuole targeting protein 7
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Phospholipid scramblase involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion. Required for mitophagy. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress. Recruits vesicle-tethering proteins TRS85 and YPT1 to the autophagosome formation site. Recruits also ATG23 and ATG8 to the PAS.
  • 基因功能參考文獻：
    1. Atg9, Stp4, and Gvp36 are putative new regulators of sphingolipid homeostasis in Saccharomyces cerevisiae. PMID: 28152593
    2. This study identified Pho23 as a master transcriptional repressor for autophagy that regulates the frequency of autophagosome formation through its negative regulation of ATG9. PMID: 24881874
    3. PAS localization of Atg9 was normal in atg27(DeltaYSAV) cells. PMID: 25557545
    4. ATG9 trafficking to phagophore assembly site depends on ATG23 and ATG27 stochiometry. PMID: 25385507
    5. TRAPPIII(trs85) is responsible for vesicular transport from early endosomes to Golgi, facilitating Atg9 cycling in autophagy. PMID: 23986483
    6. Data show the PtdIns 4-kinase Pik1 is involved in Atg9 trafficking through the Golgi and is involved in both nonselective and selective types of autophagy, whereas the PtdIns4P 5-kinase Mss4 is involved in mitophagy but not nonselective autophagy. PMID: 22977244
    7. It was shown that that the vast majority of Atg9 existed on cytoplasmic mobile vesicles that were derived from the Golgi apparatus in a process involving Atg23 and Atg27. Only a few Atg9 vesicles were needed for a single round of forming autophagosomes. PMID: 22826123
    8. Data show that membrane that contains Atg9 is delivered to the autophagosome from the Golgi-endosomal system rather than from the ER or mitochondria. PMID: 20861302
    9. The role of Atg9 trafficking in yeast and mammalian autophagy, is summarised. PMID: 20083107
    10. A different category of mutants that blocks Atg9 sorting from mitochondria, has been characterised. PMID: 16787937
    11. we demonstrate that Atg9 shuttles between the preautophagosomal structure and mitochondria PMID: 16874040
    12. Atg27 is required for Atg9 cycling and shuttles between the pre-autophagosomal structure, mitochondria, and the Golgi complex. PMID: 17135291
    13. model proposed for Atg9 cycling suggests pool of Atg11 mediates anterograde transport of Atg9 to the pre-autophagosomal structure/phagophore assembly site along the actin cytoskeleton PMID: 17329962
    14. Results support a model in which Atg9 multimerization facilitates membrane flow to the phagophore assembly site for phagophore formation. PMID: 18829864
    15. Atg9 can both promote lipid transport from the membrane origins to the phagophore assembly site, and also help assemble an intact phagophore membrane PMID: 19182520

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  • 亞細胞定位：
    Preautophagosomal structure membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein.
  • 蛋白家族：
    ATG9 family
  • 數據庫鏈接：

    KEGG: sce:YDL149W

    STRING: 4932.YDL149W



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