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  4. Recombinant Saccharomyces cerevisiae Anaphase-promoting complex subunit MND2 (MND2)

Recombinant Saccharomyces cerevisiae Anaphase-promoting complex subunit MND2 (MND2)

  • 中文名稱：
    釀酒酵母MND2重組蛋白
  • 貨號：
    CSB-YP328139SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母MND2重組蛋白
  • 貨號：
    CSB-EP328139SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母MND2重組蛋白
  • 貨號：
    CSB-EP328139SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母MND2重組蛋白
  • 貨號：
    CSB-BP328139SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母MND2重組蛋白
  • 貨號：
    CSB-MP328139SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MND2
  • Uniprot No.：
  • 別名：
    MND2; YIR025W; Anaphase-promoting complex subunit MND2; Meiotic nuclear division protein 2
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-368
  • 氨基酸序列
    MARALRDISL FNDIRKDQNS AGAKHERYNM RDLRSKKNQH VNGIDDYEDD SLDRFIRRKK SRVVKYIPSL SAYNVFNEFP YYPTSASQLL DGKLDEFLML SEQYKSRLPK IRKLGWNRFK PIGINKTMYE LEMLRSRARA QNAEGNNEED FRQHDSREED PRNNGSIGRV ILPHILQENE EYDTGEGVTG LHSMPNDSMA ILANNSANNS QNEEVSEEDE ISYDYDAEFD HVVDEDDNEE GEVPGEGVEG IEVQRERIVP DDLLMRPTSL SRSLQQFVEE AHHLDRNPYD IDSDNDGEDS KVELDMNPDF EDDVGREHDY NSEYSQEPTS YGGITPDLAS NWRNWTRERI TSLDELMERR ARQQRGQD
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication.
  • 基因功能參考文獻(xiàn)：
    1. Mnd2, a factor known to associate with APC components, prevents premature securin degradation in meiosis by APC(Ama1). PMID: 15797380
    2. The requirement of Mnd2p for anaphase-promoting complex/cyclosome function appears to be negligible under standard laboratory conditions. PMID: 15911580
    3. Data suggest that mitotic phosphorylation of Mnd2 is necessary for anaphase-promoting complex-mediated progression beyond the first meiotic nuclear division. PMID: 17459880
  • 蛋白家族：
    APC15 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YIR025W

    STRING: 4932.YIR025W



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