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  4. Recombinant Saccharomyces cerevisiae 114 kDa U5 small nuclear ribonucleoprotein component (SNU114), partial

Recombinant Saccharomyces cerevisiae 114 kDa U5 small nuclear ribonucleoprotein component (SNU114), partial

  • 中文名稱：
    釀酒酵母SNU114重組蛋白
  • 貨號：
    CSB-YP334069SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母SNU114重組蛋白
  • 貨號：
    CSB-EP334069SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母SNU114重組蛋白
  • 貨號：
    CSB-EP334069SVG-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母SNU114重組蛋白
  • 貨號：
    CSB-BP334069SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母SNU114重組蛋白
  • 貨號：
    CSB-MP334069SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SNU114
  • Uniprot No.：
  • 別名：
    SNU114; GIN10; YKL173W; YKL637Pre-mRNA-splicing factor SNU114; 114 kDa U5 small nuclear ribonucleoprotein component; Growth inhibitory protein 10
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.
  • 基因功能參考文獻(xiàn)：
    1. We propose that the U5 snRNA IL1 is a platform for protein binding and is required for , Brr2 and Snu114 association with the U5 snRNA to form the U5 snRNP. PMID: 23857713
    2. Data suggest that the RNA base pairing state is directly or indirectly sensed by the Snu114p G domain. PMID: 19620389
    3. GTP hydrolysis results in a rearrangement between Prp8 and the C terminus of Snu114 that leads to release of U1 and U4, thus activating the spliceosome for catalysis. PMID: 15911574
    4. Prp8 interaction site with Snu114 protein PMID: 16373487
    5. Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain. PMID: 16540695
    6. Snu114p serves as a signal-dependent switch that transduces signals to Brr2p to control spliceosome dynamics. PMID: 16885028
    7. Results report the structure of the Saccharomyces cerevisiae tri-snRNP, and show that U4/U6 snRNP forms a domain termed the arm, while a separate head domain adjacent to the arm harbors Brr2, and Prp8 and the GTPase Snu114 are located centrally. PMID: 18953335

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  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EF-G/EF-2 subfamily
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YKL173W

    STRING: 4932.YKL173W



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