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Recombinant Rhizobium meliloti [Protein-PII] uridylyltransferase (glnD), partial

  • 中文名稱:
    黑根瘤菌glnD重組蛋白
  • 貨號:
    CSB-YP348090RKU
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    黑根瘤菌glnD重組蛋白
  • 貨號:
    CSB-EP348090RKU
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    黑根瘤菌glnD重組蛋白
  • 貨號:
    CSB-EP348090RKU-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    黑根瘤菌glnD重組蛋白
  • 貨號:
    CSB-BP348090RKU
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    黑根瘤菌glnD重組蛋白
  • 貨號:
    CSB-MP348090RKU
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    glnD
  • Uniprot No.:
  • 別名:
    glnD; R00396; SMc01124; Bifunctional uridylyltransferase/uridylyl-removing enzyme; UTase/UR; Bifunctional [protein-PII] modification enzyme; Bifunctional nitrogen sensor protein) [Includes: [Protein-PII] uridylyltransferase; PII uridylyltransferase; UTase; EC 2.7.7.59); [Protein-PII]-UMP uridylyl-removing enzyme; UR; EC 3.1.4.-)]
  • 種屬:
    Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism.
  • 基因功能參考文獻:
    1. Either that unmodified PII proteins were responsible for the glnD mutant's ineffective phenotype or that connecting GlnD and appropriate symbiotic behavior does not require the PII proteins. PMID: 20304991
    2. These results indicate that bacterial nitrogen stress regulation is important to symbiotic productivity and suggest that GlnD may act in a novel way to influence symbiotic behavior. PMID: 19020095
  • 蛋白家族:
    GlnD family
  • 數據庫鏈接:


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