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  4. Recombinant Red clover necrotic mosaic virus RNA-directed RNA polymerase (ORF1), partial

Recombinant Red clover necrotic mosaic virus RNA-directed RNA polymerase (ORF1), partial

  • 中文名稱：
    Recombinant Red clover necrotic mosaic virus RNA-directed RNA polymerase (ORF1) ,partial
  • 貨號：
    CSB-YP328364RCB
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Red clover necrotic mosaic virus RNA-directed RNA polymerase (ORF1) ,partial
  • 貨號：
    CSB-EP328364RCB
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Red clover necrotic mosaic virus RNA-directed RNA polymerase (ORF1) ,partial
  • 貨號：
    CSB-EP328364RCB-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Red clover necrotic mosaic virus RNA-directed RNA polymerase (ORF1) ,partial
  • 貨號：
    CSB-BP328364RCB
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Red clover necrotic mosaic virus RNA-directed RNA polymerase (ORF1) ,partial
  • 貨號：
    CSB-MP328364RCB
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ORF1
  • Uniprot No.：
  • 別名：
    ORF1; RNA-directed RNA polymerase; EC 2.7.7.48; Protein p88
  • 種屬：
    Red clover necrotic mosaic virus (RCNMV)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    RNA-dependent RNA polymerase, made of isoforms p88 and p27, plays an essential role in the replication of the bipartite viral genome composed of RNA-1 and RNA-2. Replicase proteins p88 and p27 interact together and selectively recruit viral replication templates as well as other essential components into intracellular membranes for the assembly of the 480 kDa viral replicase complex. Mechanistically, isoform protein p27 binds to the Y-shaped RNA element (YRE) located in the 3' UTR of RNA2 via a direct RNA-protein interaction and to RNA1 in a translation-coupled manner. This RNA-binding activity allows RNA2 recruitment to host membranes. The viral replicase complex synthesizes the dsRNA genome from the genomic ssRNA(+). It recognizes internal subgenomic promoters on the negative-sense RNA to transcribe the 3' co-terminal subgenomic RNAs that will generate the capsid and movement proteins. In addition, the viral replication process requires the trigger of an intracellular ROS burst mediated by p27 recruitment of the host ROS-generating machinery. The viral replicase complex also acts as a suppressor of RNA-mediated gene silencing, known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs.
  • 基因功能參考文獻：
    1. A stretch of 20 amino acids in the N-terminal region of p27 is essential for the association of p27 with host plant membranes. PMID: 22898643
  • 亞細胞定位：
    [Isoform RNA-directed RNA polymerase p88]: Host endoplasmic reticulum membrane.; [Isoform protein p27]: Host endoplasmic reticulum membrane.
  • 蛋白家族：
    Tombusviridae RNA polymerase family
  • 數據庫鏈接：

    KEGG: vg:956630



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