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  4. Recombinant Rat Vesicular inhibitory amino acid transporter (Slc32a1), partial

Recombinant Rat Vesicular inhibitory amino acid transporter (Slc32a1), partial

  • 中文名稱：
    大鼠Slc32a1重組蛋白
  • 貨號：
    CSB-YP021578RA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Slc32a1重組蛋白
  • 貨號：
    CSB-EP021578RA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Slc32a1重組蛋白
  • 貨號：
    CSB-EP021578RA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Slc32a1重組蛋白
  • 貨號：
    CSB-BP021578RA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Slc32a1重組蛋白
  • 貨號：
    CSB-MP021578RA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Slc32a1; Vgat; ViaatVesicular inhibitory amino acid transporter; GABA and glycine transporter; Solute carrier family 32 member 1; Vesicular GABA transporter; rGVAT
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Involved in the uptake of GABA and glycine into the synaptic vesicles.
  • 基因功能參考文獻：
    1. cortical axon terminals co-expressing VGLUT1 and VGAT can evoke simultaneously monosynaptic glutamate and gamma-aminobutyric acid responses. PMID: 25749864
    2. Data show that VIAAT is a vesicular Cl(-) transporter that co-transports Cl(-) with GABA or glycine in a membrane-potential-dependent manner, and that Cl(-) plays an essential role in vesicular storage of GABA and glycine. PMID: 19843525
    3. VIAAT is expressed in rat islets and is more abundant in the mantle PMID: 12031963
    4. functional neuronal VIAAT is present in glucagon-containing secretory granules in pancreatic alpha-cells PMID: 12882924
    5. In mature neocortex neuronal cultures, VGLUT1 and VIAAT exhibit bidirectional and opposite regulation by prolonged activity changes. PMID: 16079394
    6. As a whole, the results provide original evidence that vGAT expression is altered in the 6-hydroxydopamine model of Parkinson's disease. PMID: 17218060
    7. Concomitantly with the loss of some subpopulations of VGAT-containing neurons, there was an up-regulation of VGAT synthesis in all remaining GABA neurons as early as 1 week after pilocarpine injection. PMID: 17503488
    8. Glycine is released from synaptic vesicles when VIAAT is coexpressed with the neural transporter GlyT2. PMID: 17554001
    9. VGLUT1 is sorted diurnally to the plasma membrane to modulate glutamate transmission during a day/night cycle, whereas VGAT expression is not oscillating but is increased in the presence of a light/dark cycle. PMID: 19103593
    10. GABAergic synapses in cortical axon terminals express both vesicular transporters VGAT and VGLUT1. PMID: 19627441

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  • 亞細胞定位：
    Cytoplasmic vesicle membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Amino acid/polyamine transporter 2 family
  • 組織特異性：
    Brain. Expressed at high levels within the neocortex, hippocampus, cerebellum, striatum, septal nuclei and the reticular nucleus of the thalamus. Also expressed in islets where it is more abundant in the peripheral/mantle region.
  • 數據庫鏈接：


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