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Recombinant Rat Vesicular acetylcholine transporter (Slc18a3), partial

  • 中文名稱:
    大鼠Slc18a3重組蛋白
  • 貨號:
    CSB-YP720309RA
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    大鼠Slc18a3重組蛋白
  • 貨號:
    CSB-EP720309RA
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    大鼠Slc18a3重組蛋白
  • 貨號:
    CSB-EP720309RA-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    大鼠Slc18a3重組蛋白
  • 貨號:
    CSB-BP720309RA
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    大鼠Slc18a3重組蛋白
  • 貨號:
    CSB-MP720309RA
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
  • Uniprot No.:
  • 別名:
    Slc18a3; Vacht; Vat; Vesicular acetylcholine transporter; VAChT; rVAT; Solute carrier family 18 member 3
  • 種屬:
    Rattus norvegicus (Rat)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Involved in acetylcholine transport into synaptic vesicles.
  • 基因功能參考文獻:
    1. VACHT is inserted into the presynaptic membrane during synaptic vesicles' exocytosis, removed during their endocytotic recycling, and plays a role in non-quantal secretion of acetylcholine. PMID: 21557989
    2. VAChT was primarily localized to membranes of small synaptic vesicles in axon terminals PMID: 11304756
    3. VAChT interacts with clathrin-associated adaptor complexes AP-1 and AP-2 PMID: 14724281
    4. Semi-quantitative ultrastructural analysis reveals that from 5% to 13% of VAChT-immunoreactive presynaptic profiles contained mu opioid receptor (MOR). Additionally, 7% of VAChT presynaptic profiles directly apposed MOR axons and terminals. PMID: 15306235
    5. This study locates the acetylcholine (ACh) binding site in the vesicular acetylcholine transporter (VAChT) and identifies the amino acid residues linked to the individual binding and rates of the transmembrane reorientation steps of a transport cycle. PMID: 15366926
    6. A model for VAChT is suggested in which the known ion pair between lysine in transmembrane domain(TMD)II and aspartate in TMDXI controls the conformation or relative position of TMDXI, which in turn controls additional TMDs in the VAChT carboxy-terminal. PMID: 15924414
    7. During VAChT trafficking, the C-terminal dileucine-containing motif is sufficient as a dual signal for both internalization and synaptic vesicle targeting. PMID: 17451554
    8. 18.0 mg/kg dose of chlorpyrifos was associated with (brain region-dependent) decreases in nerve growth factor receptors and cholinergic proteins including the vesicular acetylcholine transporter PMID: 17548533
    9. These results made [125I]-( R,R)-5-AOIBV inferior to [125I]-IBVM for the VAChT exploration in vivo. PMID: 17998100
    10. VAChT concentrations were increased in the brain of control SHR compared to age-matched normotensive Wistar-Kyoto rats. PMID: 19021024
    11. Sympathetic preganlionics segregate mENK from VAChT and ChAT; most of the subset of mENKergic varicosities negative for VAChT also do not express Syn, suggesting a different vesicular pattern in these sympathetic preganglionic varicosities. PMID: 19524025

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  • 亞細胞定位:
    Membrane; Multi-pass membrane protein.
  • 蛋白家族:
    Major facilitator superfamily, Vesicular transporter family
  • 組織特異性:
    High expression in all major cholinergic cell groups, including peripheral postganglionic parasympathetic cells, preganglionic sympathetic and parasympathetic cells, ventral spinal cord and brainstem motoneurons, cell groups in the basal forebrain, the ha
  • 數據庫鏈接:

    KEGG: rno:60422

    UniGene: Rn.9987



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