Recombinant Rat Type II iodothyronine deiodinase (Dio2)

中文名稱：

大鼠Dio2重組蛋白
貨號：

CSB-YP302542RA
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Dio2
Uniprot No.：

P70551
別名：

Dio2; Itdi2; Txdi2Type II iodothyronine deiodinase; EC 1.21.99.4; 5DII; DIOII; Type 2 DI; Type-II 5'-deiodinase
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Full Length
來源：

Yeast
分子量：

31.8kDa
表達區域：

1-266aa
氨基酸序列

MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHVALLLSRSKSTRGEWRRMLTSEGLRCVWNSFLLDAYKQVKLGEDAPNSSVVHVSNPEAGNNCASEKTADGAECHLLDFASAERPLVVNFGSATUPPFTRQLPAFRQLVEEFSSVADFLLVYIDEAHPSDGWAVPGDSSMSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADRMDNNANVAYGVAFERVCIVQRRKIAYLGGKGPFSYNLQEVRSWLEKNFSKRUILD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Catalyzes the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.
基因功能參考文獻：
1. Glucocorticoids inhibited the adrenergic stimulation of Dio2 at the transcriptional level in brown adipocytes. PMID: 26994513
2. This study demonstrated that reduction of the Dio2 mRNA expression in hippocampus of thyroidectomized Wistar rats. PMID: 26861177
3. These studies reveal that tissue-specific differences in D2 ubiquitination are an inherent property of the TRH/TSH feedback mechanism PMID: 25555216
4. cold exposure is accompanied by concerted changes in the metabolism of BAT and oxidative and glycolytic skeletal muscles that are paralleled by type 2 deiodinase activation PMID: 25294216
5. results of the present study hypothesize that progesterone withdrawal may underlie the decrement in D2 expression, with consequent reduction in the peripheral conversion of T4 into T3 leading to a hypothyroid state. PMID: 24362948
6. Data suggest that type 2 deiodinase is induced in inflammation of tanycytes (as in inflammation of periventricular area, arcuate nucleus, and median eminence of hypothalamus); this enzyme induction appears to involve NFkappaB signaling. PMID: 24635351
7. Data suggest that type 2 deiodinase induction (and kinetics of induction) in animal models of inflammation of leptomeninges, choroid plexus, and brain blood vessels is tissue specific and species specific. PMID: 24601886
8. In rat articular cartilage, DIO2 is specifically expressed among deiodinases and dominantly expressed the same as in brown adipose tissue. PMID: 23296253
9. Data indicate that low-iodine diets induces changes in deiodinase activities, especially in the thyroid, to counteract the low T(4) synthesis and secretion, contributing to maintain the local T(3) concentrations in the tissues with D2 activity. PMID: 23142811
10. Results indicate that, beside activation of CREB, epigenetic factors such as histone modifications also play an important role in regulating Dio2 transcription. PMID: 21704117
11. T3 increases the adrenergic stimulation of UCP1 and D2 expression mostly via the TRbeta1 isoform, and in brown adipocytes, D2 is protected from degradation by the action of T3 on TRbeta1. PMID: 20719854
12. In addition to TSH, other factors derived from the pars tuberalis may contribute to LPS-induced D2 activation in tanycytes. PMID: 20501675
13. Triiodothyronine is required for the stimulation of type II 5'-deiodinase mRNA in rat brown adipocytes. PMID: 11934678
14. induction of thyroid hormone-degrading deiodinase in cardiac hypertrophy and failure PMID: 12072417
15. data do not support that thyroxine-binding protein p29 has a functional relationship with D2 PMID: 12586781
16. D2 activity not induced by decidualization stimulus. In spontaneously cycling female rats, both D2 and D3 were observed to be 3- to 8-fold higher in proestrus, compared with diestrus. PMID: 12959985
17. the suprachiasmatic nuclei separately stimulates D2 activity as well as the hypothalamo-pituitary-thyroid axis PMID: 15550511
18. MAPK signaling is required for the adrenergic stimulation of D2 activity and mRNA PMID: 15691884
19. appropriate induction of Dio2 activity during negative energy balance is dependent upon both leptin and glucocorticoid signaling. PMID: 15746256
20. Hypoxia leads to stabilization of D2 by slowing its degradation by the proteasome pathway. PMID: 17615150
21. Correlation between relative mRNA levels of WSB-1 and USP-33 in numerous tissues that do not express type 2 deiodinase(D2) suggests that these ubiquitin-related enzymes share additional substrates besides D2. PMID: 17628004
22. Induction of type 2 iodothyronine deiodinase in the mediobasal hypothalamus by bacterial lipopolysaccharide. PMID: 18218695
23. The proteins encoded by rat and human Dio2 (deiodinase, iodothyronine, type II) genes are selenoproteins. PMID: 8755651
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亞細胞定位：

Membrane; Single-pass membrane protein.
蛋白家族：

Iodothyronine deiodinase family
組織特異性：

Expressed in cerebral cortex, cerebellum, pituitary gland, mostly in anterior pituitary gland, and pineal gland, as well as in brown adipose tissue (BAT).
數據庫鏈接：

KEGG: rno:65162

UniGene: Rn.88380