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  4. Recombinant Rat Type I iodothyronine deiodinase (Dio1), partial

Recombinant Rat Type I iodothyronine deiodinase (Dio1), partial

  • 中文名稱：
    大鼠Dio1重組蛋白
  • 貨號：
    CSB-YP340619RA1
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Dio1重組蛋白
  • 貨號：
    CSB-EP340619RA1
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Dio1重組蛋白
  • 貨號：
    CSB-EP340619RA1-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Dio1重組蛋白
  • 貨號：
    CSB-BP340619RA1
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Dio1重組蛋白
  • 貨號：
    CSB-MP340619RA1
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Dio1
  • Uniprot No.：
  • 別名：
    Dio1; Itdi1; Txdi1; Type I iodothyronine deiodinase; 5DI; DIOI; Type 1 DI; Type-I 5'-deiodinase
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine).
  • 基因功能參考文獻：
    1. estrogen deficiency impairs the induction of thyroid hormone activating enzyme D1 in the pituitary, and GH release by acute exercise. Also, acute D1 activation is essential for exercise-induced GH response PMID: 25874614
    2. Response of pituitary Dio1 and brown adipose tissue Dio2 is blunted in ovariectomized rats. PMID: 22815055
    3. The effects of androgen deprivation on thyroid homeostasis, reflected in DioI and DioII expression, are reported. PMID: 22956722
    4. Type 1 and type 2 deiodinase may have distinct functions in white adipose tissue, regulating lipogenesis and lipolysis. PMID: 21397282
    5. The study provided first evidence on the reduction of liver RAR concentrations and 5'-DI activity in response to long-term voluntary wheel running. PMID: 19646447
    6. Mammary gland carcinoma-related increase of type I iodothyronine 5'-deiodinase activity in Sprague-Dawley rats. PMID: 11765219
    7. D1 3'UTR may play an important role in regulating the stability and translational efficiency of the D1 mRNA PMID: 16230777
    8. The effect of neonatal thyroxine treatment on body weight, fat proportion, serum leptin, and thyroid function of 60-day-old rats, is evaluated. PMID: 18197581
    9. Epididymis expresses the highest 5'-deiodinase activity in the male reproductive system. PMID: 18467445
    10. The results demonstrate that decreased liver D1 and BAT D2 might be involved in the decreased T(4)-to-T(3) conversion detected after an exercise session on the treadmill. PMID: 18539729

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  • 亞細胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein.
  • 蛋白家族：
    Iodothyronine deiodinase family
  • 組織特異性：
    Highly expressed in the liver, kidney and thyroid gland of adult rats.
  • 數據庫鏈接：

    KEGG: rno:25430

    UniGene: Rn.87549



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