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  4. Recombinant Rat Ras-related protein Rab-27A (Rab27a)

Recombinant Rat Ras-related protein Rab-27A (Rab27a)

  • 中文名稱：
    大鼠Rab27a重組蛋白
  • 貨號：
    CSB-YP019177RA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Rab27a重組蛋白
  • 貨號：
    CSB-EP019177RA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Rab27a重組蛋白
  • 貨號：
    CSB-EP019177RA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Rab27a重組蛋白
  • 貨號：
    CSB-BP019177RA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Rab27a重組蛋白
  • 貨號：
    CSB-MP019177RA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Rab27a
  • Uniprot No.：
  • 別名：
    Rab27a; Rab27; Ras-related protein Rab-27A; Rab-27; EC 3.6.5.2; GTP-binding protein Ram; Ram p25
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Full length protein
  • 表達區域：
    1-221
  • 氨基酸序列
    MSDGDYDYLI KFLALGDSGV GKTSVLYQYT DGKFNSKFIT TVGIDFREKR VVYRANGPDG TVGRGQRIHL QLWDTAGQER FRSLTTAFFR DAMGFLLLFD LTNEQSFLNV RNWISQLQMH AYCENPDIVL CGNKSDLEDQ RAVKEEEARE LAEKYGIPYF ETSAANGTNI SQAIEMLLDL IMKRMERCVD KSWIPEGVVR SNGHTSTDQL SEEKEKGLCG C
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
  • 基因功能參考文獻：
    1. Data show that the intracellular M4M5 loop of human alpha1 subunit of Na+,K+-ATPase (ATP1a1) interacts directly with rat rab GTP-binding protein Rab27a. PMID: 25051489
    2. These findings indicated that Madd functions as a guanine nucleotide exchange factor for Rab27 in parotid acinar cells and regulates isoproterenol-stimulated amylase release. PMID: 23702376
    3. cyt b(558) depends on clathrin for internalization, and in activated macrophages NADPH oxidase occupies a Rab27A/B-regulated secretory compartment PMID: 22157766
    4. Rab27A interacts with Rabphilin and Noc2 which are recruited to dense-core vesicles in rat cells PMID: 14722103
    5. Results demonstrate that Rab27A plays a crucial role in antigen-induced histamine release from RBL-2H3 cells. PMID: 15465017
    6. rab27a is a direct partner of munc13-4. The complex of the two proteins is an essential regulator of secretory granule fusion with the plasma membrane in hematopietic cells. Mutations in either of the two genes prevent formation of this compex. PMID: 15548590
    7. Our results suggest that the Noc2/Rab27 complex is an important constituent of the early stages of IPR-stimulated amylase release. PMID: 17067543
    8. Three Rab3/27 effectors, Granuphilin, Noc2, and Rabphilin, in PC12 cells using fluorescence recovery after photobleaching of EGFP-tagged proteins. PMID: 18573236

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  • 亞細胞定位：
    Membrane; Lipid-anchor. Melanosome. Late endosome. Lysosome.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 組織特異性：
    High levels in eye, intestine, lung, pancreas and spleen, and low or absent in brain, liver, heart, kidney, and skeletal muscle.
  • 數據庫鏈接：


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