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  4. Recombinant Rat Poly (U)-binding-splicing factor PUF60 (Puf60)

Recombinant Rat Poly (U)-binding-splicing factor PUF60 (Puf60)

  • 中文名稱：
    大鼠Puf60重組蛋白
  • 貨號：
    CSB-YP892996RA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Puf60重組蛋白
  • 貨號：
    CSB-EP892996RA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Puf60重組蛋白
  • 貨號：
    CSB-EP892996RA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Puf60重組蛋白
  • 貨號：
    CSB-BP892996RA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Puf60重組蛋白
  • 貨號：
    CSB-MP892996RA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Puf60
  • Uniprot No.：
  • 別名：
    Puf60; Siahbp1; Poly(U)-binding-splicing factor PUF60; 60 kDa poly(U)-binding-splicing factor; RNA-binding protein Siah-BP; Siah-binding protein 1
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-564
  • 氨基酸序列
    MATATIALQV NGQQGGGSEP AAAAAAAAAA VVAAGDKWKP PQGTESIKME NGQSTGTKLG LPPLTPEQQE ALQKAKKYAM EQSIKSVLVK QTIAHQQQQL TNLQMAAVTM GFGDPLSPLQ SMAAQRQRAL AIMCRVYVGS IYYELGEDTI RQAFAPFGPI KSIDMSWDSV TMKHKGFAFV EYEVPEAAQL ALEQMNSVML GGRNIKVGRP SNIGQAQPII DQLAEEARAF NRIYVASVHQ DLSDDDIKSV FEAFGKIKSC TLARDPTTGK HKGYGFIEYE KAQSSQDAVS SMNLFDLGGQ YLRVGKAVTP PMPLLTPATP GGLPPAAAVA AAAATAKITA QEAVAGAAVL GTLATPGLVS PALTLAQPLG ALPQAVMAAQ APGVITGVTP ARPPIPVTIP SVGVVNPILA SPPTLGLLEP KKEKEEEELF PESERPEMLS EQEHMSISGS SARHMVMQKL LRKQESTVMV LRNMVDPKDI DDDLEGEVTE ECGKFGAVNR VIIYQEKQGE EEDAEIIVKI FVEFSMASET HKAIQALNGR WFGGRKVVAE VYDQERFDNS DLSA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    RRM half pint family
  • 數據庫鏈接：


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