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Recombinant Rat Phosducin (Pdc)

  • 中文名稱:
    Recombinant Rat Phosducin (Pdc)
  • 貨號:
    CSB-EP017663RA
  • 規(guī)格:
    ¥1344
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產(chǎn)品詳情

  • 純度:
    Greater than 85% as determined by SDS-PAGE.
  • 生物活性:
    Not Test
  • 基因名:
    Pdc
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 蛋白長度:
    Full Length
  • 來源:
    E.coli
  • 分子量:
    35.0 kDa
  • 表達區(qū)域:
    1-246aa
  • 氨基酸序列
    MEEAASQSLEEDFEGQATHTGPKGVINDWRKFKLESEDGDSIPPSKKEILRQMSSPQSRDDKDSKERMSRKMSIQEYELIHQDKEDEGCLRKYRRQCMQDMHQKLSFGPRYGFVYELETGEQFLETIEKEQKVTTIVVNIYEDGVRGCDALNSSLECLAAEYPMVKFCKIRASNTGAGDRFSSDVLPTLLVYKGGELISNFISVAEQFAEDFFAADVESFLNEYGLLPEREIHDLGQTNTEDEDIE
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    C-terminal 6xHis-tagged
  • 產(chǎn)品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能:
    Inhibits the transcriptional activation activity of the cone-rod homeobox CRX. May participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism.
  • 基因功能參考文獻:
    1. 14-3-3 protein-mediated inhibition of Pdc binding to Gtbetagamma is based on steric occlusion of Pdc's Gtbetagamma binding surface. PMID: 28402877
    2. C-terminal domain of Pdc interacts with the outside surface of the 14-3-3 dimer. PMID: 25971962
    3. 14-3-3 phosphorylation of Pdc at Ser-54 and Ser-73 affects the structure of the whole Pdc molecule. PMID: 23199924
    4. data suggest that phosducin action is based on the reduction of transducin affinity to the membranes of rod outer segments, achieved by keeping the transducin beta gamma subunits apart from the alpha subunit PMID: 14973130
    5. phosducin has a role in regulating signaling in the rod outer segment compartment and there are distinct functions for phosphorylation sites 54 and 71 PMID: 17569665

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  • 亞細胞定位:
    Cytoplasm, cytosol. Nucleus. Cell projection, cilium, photoreceptor outer segment. Photoreceptor inner segment.
  • 蛋白家族:
    Phosducin family
  • 數(shù)據(jù)庫鏈接:


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