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  4. Recombinant Rat Phosducin (Pdc)

Recombinant Rat Phosducin (Pdc)

  • 中文名稱：
    大鼠Pdc重組蛋白
  • 貨號：
    CSB-YP017663RA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Pdc重組蛋白
  • 貨號：
    CSB-EP017663RA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Pdc重組蛋白
  • 貨號：
    CSB-BP017663RA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Pdc重組蛋白
  • 貨號：
    CSB-MP017663RA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Pdc
  • Uniprot No.：
  • 別名：
    Pdc; Rpr1; Phosducin; PHD; 33 kDa phototransducing protein; Protein MEKA; Rod photoreceptor 1; RPR-1
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Full length protein
  • 表達區域：
    1-246
  • 氨基酸序列
    MEEAASQSLE EDFEGQATHT GPKGVINDWR KFKLESEDGD SIPPSKKEIL RQMSSPQSRD DKDSKERMSR KMSIQEYELI HQDKEDEGCL RKYRRQCMQD MHQKLSFGPR YGFVYELETG EQFLETIEKE QKVTTIVVNI YEDGVRGCDA LNSSLECLAA EYPMVKFCKI RASNTGAGDR FSSDVLPTLL VYKGGELISN FISVAEQFAE DFFAADVESF LNEYGLLPER EIHDLGQTNT EDEDIE
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Inhibits the transcriptional activation activity of the cone-rod homeobox CRX. May participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism.
  • 基因功能參考文獻：
    1. 14-3-3 protein-mediated inhibition of Pdc binding to Gtbetagamma is based on steric occlusion of Pdc's Gtbetagamma binding surface. PMID: 28402877
    2. C-terminal domain of Pdc interacts with the outside surface of the 14-3-3 dimer. PMID: 25971962
    3. 14-3-3 phosphorylation of Pdc at Ser-54 and Ser-73 affects the structure of the whole Pdc molecule. PMID: 23199924
    4. data suggest that phosducin action is based on the reduction of transducin affinity to the membranes of rod outer segments, achieved by keeping the transducin beta gamma subunits apart from the alpha subunit PMID: 14973130
    5. phosducin has a role in regulating signaling in the rod outer segment compartment and there are distinct functions for phosphorylation sites 54 and 71 PMID: 17569665

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  • 亞細胞定位：
    Cytoplasm, cytosol. Nucleus. Cell projection, cilium, photoreceptor outer segment. Photoreceptor inner segment.
  • 蛋白家族：
    Phosducin family
  • 數據庫鏈接：


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