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  4. Recombinant Rat Otoferlin (Otof), partial

Recombinant Rat Otoferlin (Otof), partial

  • 中文名稱：
    大鼠Otof重組蛋白
  • 貨號：
    CSB-YP017272RA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Otof重組蛋白
  • 貨號：
    CSB-EP017272RA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Otof重組蛋白
  • 貨號：
    CSB-EP017272RA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Otof重組蛋白
  • 貨號：
    CSB-BP017272RA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Otof重組蛋白
  • 貨號：
    CSB-MP017272RA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Otof; Fer1l2; Otoferlin; Fer-1-like protein 2
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Interacts in a calcium-dependent manner to the presynaptic SNARE proteins at ribbon synapses of cochlear inner hair cells (IHCs) to trigger exocytosis of neurotransmitter. Also essential to synaptic exocytosis in immature outer hair cells (OHCs). May also play a role within the recycling of endosomes.
  • 基因功能參考文獻：
    1. otoferlin, through GAD65, modulates GABAergic activity. PMID: 25701657
    2. a direct role for otoferlin in exocytosis and modulation of calcium-dependent membrane fusion PMID: 24478316
    3. otoferlin-AP-2 interaction drives Ca(2+)- and stimulus-dependent compensating clathrin-mediated endocytosis in mature inner hair PMID: 23719817
    4. Otof CA domain differs structurally and functionally from other C(2) domains PMID: 21216247
    5. Otoferlin mRNA and protein was detected in mature outer hair cells of low-frequency processing cochlear turns and in auditory nerve fibres. In outer, inner and vestibular hair cells PMID: 17229086
    6. Using a rodent cochlear cDNA library Rab8b is identified as an interacting partner of otoferlin. PMID: 18772196

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  • 亞細胞定位：
    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type II membrane protein. Basolateral cell membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Cell junction, synapse, presynaptic cell membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Ferlin family
  • 組織特異性：
    Isoform 1 is expressed in the cochlea and brain. Expressed in cerebellum (Purkinje cells), hippocampus (granule cells of the dentate gyrus and in pyramidal cells of the CA1-CA3 region) and cortex (stellate and pyramidal cells). Expressed in hair cells of
  • 數據庫鏈接：


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