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  4. Recombinant Rat Neurabin-1 (Ppp1r9a), partial

Recombinant Rat Neurabin-1 (Ppp1r9a), partial

  • 中文名稱：
    大鼠Ppp1r9a重組蛋白
  • 貨號：
    CSB-YP018557RA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Ppp1r9a重組蛋白
  • 貨號：
    CSB-EP018557RA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Ppp1r9a重組蛋白
  • 貨號：
    CSB-EP018557RA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Ppp1r9a重組蛋白
  • 貨號：
    CSB-BP018557RA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Ppp1r9a重組蛋白
  • 貨號：
    CSB-MP018557RA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Ppp1r9a
  • Uniprot No.：
  • 別名：
    Ppp1r9a; Neurabin-1; Neurabin-I; Neural tissue-specific F-actin-binding protein I; PP1bp175; Protein phosphatase 1 regulatory subunit 9A; p180
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May be involved in neurite formation. Inhibits protein phosphatase 1-alpha activity. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction.
  • 基因功能參考文獻：
    1. Data show that the PP1:spinophilin holoenzyme is dynamic in solution, which allows for an increased capture radius of spinophilin and is likely important for its biological role. PMID: 21094159
    2. The spinophilin interactome was charaterized in subcellular fractions solubilized from adult rodent striatum. PMID: 20124353
    3. recruit specific isoforms of protein phosphatase-1 catalytic subunits PMID: 12016225
    4. N-terminal F-actin-binding domain dictated neurabin I localization at actin cytoskeleton and promoted disassembly of stress fibers (neurabin I) PMID: 12052877
    5. there is a novel PP1gamma1-selective interaction domain in neurabin that may allow for selective regulation and/or subcellular targeting of PP1 isoforms PMID: 15016827
    6. Postsynaptic actin-bound neurabin-I-PP1 complex regulates synaptic transmission and bidirectional changes in hippocampal plasticity. PMID: 16899074
    7. NrbI-targeted PP1, in response to distinct synaptic activities, regulates the synaptic trafficking of specific AMPAR subunits PMID: 17460080
    8. study determined the structure of the neurabin SAM domain by solution state nuclear magnetic resonance spectroscopy PMID: 17600833
    9. Mutation of the Cdk5 phosphorylation site reduces the phenotypic consequences of Neurabin-I overexpression both in vitro and in vivo PMID: 17699587
    10. selective targeting of PP1gamma1 to F-actin by neurabins 1& 2 in intact cells requires both the canonical PP1-binding motif and an auxiliary PP1gamma1-selectivity determinant PMID: 18216290

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton. Cell junction, synapse, synaptosome.
  • 組織特異性：
    Brain, and widely expressed in neural tissue. Highly concentrated in synapses of developed neurons. In developing neurons, concentrated in the lamellipodia of the growth cone.
  • 數據庫鏈接：


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