Recombinant Rat Methylated-DNA--protein-cysteine methyltransferase (Mgmt)

中文名稱：

大鼠Mgmt重組蛋白
貨號(hào)：

CSB-YP013788RA
規(guī)格：
來源：

Yeast
其他：

中文名稱：

大鼠Mgmt重組蛋白
貨號(hào)：

CSB-EP013788RA
規(guī)格：
來源：

E.coli
其他：

中文名稱：

大鼠Mgmt重組蛋白
貨號(hào)：

CSB-EP013788RA-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大鼠Mgmt重組蛋白
貨號(hào)：

CSB-BP013788RA
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

大鼠Mgmt重組蛋白
貨號(hào)：

CSB-MP013788RA
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Mgmt
Uniprot No.：

P24528
別名：

MgmtMethylated-DNA--protein-cysteine methyltransferase; EC 2.1.1.63; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase
種屬：

Rattus norvegicus (Rat)
蛋白長(zhǎng)度：

Full length protein
表達(dá)區(qū)域：

1-209
氨基酸序列

MAEICKMKYT VLDSPLGKIE LSGCERGLHG IRFLSGKTPN TDPTEAPACP EVLGGPEGVP EPLVQCTAWL EAYFHEPAAT EGLPLPALHH PVFQQDSFTR QVLWKLLKVV KFGEMVSYQQ LAALAGNPKA ARAVGGAMRS NPVPILIPCH RVIRSDGAIG NYSGGGQTVK EWLLAHEGIP TGQPASKGLG LIGSWLKPSF ESSSPKPSG
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
基因功能參考文獻(xiàn)：
1. Folate deficiency resulted in significant increases in levels of the DNA repair proteins OGG-1 and MGMT in the liver PMID: 20051376
2. Divalent cations stabilized rat recombinant O6-methylguanine-DNA methyltransferase (rMGMT) protein against heat treatment PMID: 12479403
3. age-related differences in MGMT activity may play a significant role in age-differential susceptibility to rat mammary gland and kidney carcinogenesis PMID: 16086375
亞細(xì)胞定位：

Nucleus.
蛋白家族：

MGMT family
數(shù)據(jù)庫鏈接：

KEGG: rno:25332

STRING: 10116.ENSRNOP00000021537

UniGene: Rn.9836