Recombinant Rat Dystrophin (Dmd), partial

中文名稱：

大鼠Dmd重組蛋白
貨號：

CSB-EP006963RA
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Dmd
Uniprot No.：

P11530
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Partial
來源：

E.coli
分子量：

35.0 kDa
表達區域：

1-240aa
氨基酸序列

MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIDNLFSDLQDGKRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALQVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKTIMAGLQQTNSEKILLSWVRESTRNYPQVNVLNFTSSWSDGLALNALIHSHRPDLFDWNSVVSQQSATQRLEHAFNIAKCQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin-associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission.
基因功能參考文獻：
1. that decreased expression of dystrophin and increased expression of calpains are coincident and could work as possible therapeutic targets to prevent heart failure as a consequence of cardiac hypertrophy PMID: 29267303
2. results show that dystrophin 71d, dystrophin 71f and dystrophin-associated protein integrate protein complexes, and both complexes were associated to nuclear speckles structures. PMID: 26378780
3. Dp40 can interact with beta-dystroglycan as observed by co-localization assays and the co-localization was reduced with Dp40 mutants. PMID: 26004254
4. Hypoxia-induced muscle damage is not accompanied by the loss of dystrophin and desmin. PMID: 20717635
5. Dp71 is a component of the mitotic spindle and cytokinesis multi-protein apparatuses that might modulate the cell division cycle by affecting lamin B1 and beta-dystroglycan levels. PMID: 21886794
6. This study found that cells stably expressing Dp71Delta(78-79) (PC12-C11) differentiate more efficiently than non-transfected cells. PMID: 21668890
7. we established by immunoprecipitation assays that Dp71 and the dystrophin-associated proteins form a dystrophin-associated protein complex in the cell nucleus. PMID: 19784870
8. dysfunction of dystrophin may result in endothelial and astroglial damage leading to vasogenic edema that is involved in pathogenesis of epileptogenesis PMID: 21029730
9. SAP97 and dystrophin macromolecular complexes determine two pools of cardiac sodium channels Nav1.5 in cardiomyocytes. PMID: 21164104
10. results reveal a crucial role of MMP-mediated beta-DG cleavage in autoimmune neuritis, such as Guillain-Barre' syndrome, and bring insights into therapeutic strategies for autoimmune diseases. PMID: 20097170
11. Components of dystrophin-dystroglycan complex were also distributed along the blood vessels of the pineal gland PMID: 19924636
12. the distribution of dystrophin in series-fibred muscles suggests they are involved in transmission of tension from PMID: 12037582
13. expression of dystrophin during skeletal muscle regeneration PMID: 12086334
14. During a cycle of regeneration in tibialis anterior muscle following myonecrosis, dystrophin is found to be weakly labeled at the sarcolemma on day 5 and moderately stained on day 7. PMID: 12416719
15. Loss of sarcolemmal dystrophin may be involved in the mechanism of contractile force-induced reperfusion injury. PMID: 12890920
16. Results present immunocytochemical localization of beta-dystroglycan, the central member of the dystrophin glycoprotein complex, and show that beta-dystroglycan and utrophin are colocalized in clusters in all parts of Muller cells. PMID: 14711029
17. Cardioprotection conferred by ischemic preconditioning may be enhanced by temporary blockade of contractile activity until restoration of membrane dystrophin during reperfusion. PMID: 15001448
18. Dmd deficient PC12 cells generated by addition of antisense-Dp71 cells exhibited a marked suppression of neurite outgrowth. Early responses were normal, late responses were disturbed. PMID: 15149856
19. Dp71 protein contributes to the proper functioning of the cerebrospinal fluid and blood-brain barriers PMID: 15501597
20. These data support the notion of a physiological role of Dp71f distinct from other dystrophin isoforms present in the central nervous system. PMID: 16841465
21. evaluation of the effect of repeated bouts of exercise on on skeletal muscle titin, desmin & dystrophin; desmin and dystrophin mRNA levels were increased after the first and fifth sessions PMID: 17436058
22. Dp71f modulates GSK3-beta recruitment to the beta1-integrin adhesion complex. PMID: 18677563
23. Dp71d is a dynamic component of nuclear matrix that might participate in the nuclear modeling occurring during neuronal differentiation. PMID: 18687308
24. Findings demonstrate the interdependence between laminin binding to dystroglycan and GM1-containing lipid raft reorganization and provide novel insight into the dystrophin complex regulation of AQP4 polarization in astrocytes. PMID: 19451651
25. dystrophin Dp71f is important for the function that the beta1-integrin complex has during neurite outgrowth PMID: 19681445
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亞細胞定位：

Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell junction, synapse, postsynaptic cell membrane.
組織特異性：

Strongly expressed in skeletal muscle and weak expression observed in newborn brain which increases in adult brain.
數據庫鏈接：

STRING: 10116.ENSRNOP00000029969

UniGene: Rn.10307