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  4. Recombinant Rat Cysteine-rich secretory protein 1 (Crisp1)

Recombinant Rat Cysteine-rich secretory protein 1 (Crisp1)

  • 中文名稱：
    Recombinant Rat Cysteine-rich secretory protein 1(Crisp1)
  • 貨號：
    CSB-YP005974RA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Rat Cysteine-rich secretory protein 1(Crisp1)
  • 貨號：
    CSB-EP005974RA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Rat Cysteine-rich secretory protein 1(Crisp1)
  • 貨號：
    CSB-EP005974RA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Rat Cysteine-rich secretory protein 1(Crisp1)
  • 貨號：
    CSB-BP005974RA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Rat Cysteine-rich secretory protein 1(Crisp1)
  • 貨號：
    CSB-MP005974RA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Crisp1
  • Uniprot No.：
  • 別名：
    Crisp1; AegCysteine-rich secretory protein 1; 32 kDa epididymal protein; Acidic epididymal glycoprotein; Protein D; Protein E; Protein IV; Sialoprotein; Sperm-coating glycoprotein; SCP
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區(qū)域：
    20-246
  • 氨基酸序列
    Q DTTDEWDRDL ENLSTTKLSV QEEIINKHNQ LRRTVSPSGS DLLRVEWDHD AYVNAQKWAN RCIYNHSPLQ HRTTTLKCGE NLFMANYPAS WSSVIQDWYD ESLDFVFGFG PKKVGVKVGH YTQVVWNSTF LVACGVAECP DQPLKYFYVC HYCPGGNYVG RLYSPYTEGE PCDSCPGNCE DGLCTNSCEY EDNYSNCGDL KKMVSCDDPL LKEGCRASCF CEDKIH
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    This protein is supposed to help spermatozoa undergo functional maturation while they move from the testis to the ductus deferens.
  • 基因功能參考文獻：
    1. involvement of CRISP1-Zn(2+) complexes in the association of the loosely bound population of CRISP1 with sperm during epididymal maturation PMID: 21593480
    2. In addition to its influence on protein tyrosine phosphorylation, bicarbonate is required to support other rat sperm capacitation-associated events, such as migration of CRISP-1 to equatorial segment, and expression of ability of sperm to fuse with egg. PMID: 14711787
    3. the egg-binding ability of CRISP1 resides within a 45-amino acid N-terminal region containing the two motifs of the CRISP family named Signature 1 and Signature 2, the binding site of CRISP1 resides in an evolutionarily conserved region PMID: 16872593
    4. Recombinant Crisp1 is able to elicit a specific immune response and inhibit male and female fertility, supporting the use of this sperm epididymal protein for the development of an immunocontraceptive approach. PMID: 17482178
    5. CRISP1 participates not only in sperm-egg fusion but also in the prior stage of sperm-ZP interaction. PMID: 17671267
    6. CRISP1 interacts with sperm transiently, possibly with a specific receptor on the sperm surface, consistent with its action in suppressing capacitation during epididymal storage of sperm PMID: 18703418

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    CRISP family
  • 組織特異性：
    Secreted by the epididymal epithelium and then becomes associated with the sperm surface.
  • 數(shù)據(jù)庫鏈接：


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