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  4. Recombinant Rabbit Creatine kinase M-type (CKM)

Recombinant Rabbit Creatine kinase M-type (CKM)

  • 中文名稱：
    Recombinant Rabbit Creatine kinase M-type(CKM)
  • 貨號：
    CSB-YP005459RB
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Rabbit Creatine kinase M-type(CKM)
  • 貨號：
    CSB-EP005459RB
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Rabbit Creatine kinase M-type(CKM)
  • 貨號：
    CSB-EP005459RB-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Rabbit Creatine kinase M-type(CKM)
  • 貨號：
    CSB-BP005459RB
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Rabbit Creatine kinase M-type(CKM)
  • 貨號：
    CSB-MP005459RB
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    CKM
  • Uniprot No.：
  • 別名：
    CKM; Creatine kinase M chain; Creatine kinase M-type; KCRM_RABIT; M-CK
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-381
  • 氨基酸序列
    PFGNTHNKY KLNYKSEEEY PDLSKHNNHM AKVLTPDLYK KLRDKETPSG FTLDDVIQTG VDNPGHPFIM TVGCVAGDEE SYTVFKDLFD PIIQDRHGGF KPTDKHKTDL NHENLKGGDD LDPHYVLSSR VRTGRSIKGY TLPPHCSRGE RRAVEKLSVE ALNSLTGEFK GKYYPLKSMT EQEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKSFLVWVN EEDHLRVISM EKGGNMKEVF RRFCVGLQKI EEIFKKAGHP FMWNEHLGYV LTCPSNLGTG LRGGVHVKLA HLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GSVFDISNAD RLGSSEVEQV QLVVDGVKLM VEMEKKLEKG QSIDDMIPAQ K
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable).
  • 基因功能參考文獻：
    1. water molecules affect protein conformation around residue 268, thereby influencing protein stability at low temperature PMID: 24940915
    2. role of linker on the isolated C-terminal domain of rabbit muscle creatine kinase PMID: 22947872
    3. when gly(268) in rabbit muscle-specific creatine kinase was substituted with asn(268), the rabbit muscle-specific CK G286N mutant specific activity at pH 8.0 and 10 degrees C was more than 2-fold higher than that in the wild-type rabbit enzyme PMID: 21130895
    4. Creatine kinase binds more firmly to the M-band of rabbit skeletal muscle myofibrils in the presence of its substrates. PMID: 17578655
    5. In addition to its role in the in vivo functions, the linker also played a crucial role in the stability and folding of CKM. PMID: 17616428
    6. Changing the substrate specificity of creatine kinase from creatine to glycocyamine. PMID: 17976392
    7. There are three conformations of CK arranged according to the "energy minimizing principle" by ligated substrates. PMID: 19049907
    8. These results suggested that the electrostatic interactions were crucial to the action of the linker in CK reactivation. PMID: 19263506
    9. Study work provides evidence that the presence at the air/water interface of discrete domains with increased charge density, may lead to difference in partition of soluble proteins such as mtCK, interacting with the lipid monolayer. PMID: 19341780

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  • 蛋白家族：
    ATP:guanido phosphotransferase family
  • 數據庫鏈接：

    KEGG: ocu:100009056

    UniGene: Ocu.6249



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