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  4. Recombinant Pyrococcus kodakaraensis Probable L-threonine 3-dehydrogenase (tdh)

Recombinant Pyrococcus kodakaraensis Probable L-threonine 3-dehydrogenase (tdh)

  • 中文名稱(chēng)：
    Recombinant Pyrococcus kodakaraensis Probable L-threonine 3-dehydrogenase(tdh)
  • 貨號(hào)：
    CSB-YP702481FHY
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    Recombinant Pyrococcus kodakaraensis Probable L-threonine 3-dehydrogenase(tdh)
  • 貨號(hào)：
    CSB-EP702481FHY
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱(chēng)：
    Recombinant Pyrococcus kodakaraensis Probable L-threonine 3-dehydrogenase(tdh)
  • 貨號(hào)：
    CSB-EP702481FHY-B
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    Recombinant Pyrococcus kodakaraensis Probable L-threonine 3-dehydrogenase(tdh)
  • 貨號(hào)：
    CSB-BP702481FHY
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    Recombinant Pyrococcus kodakaraensis Probable L-threonine 3-dehydrogenase(tdh)
  • 貨號(hào)：
    CSB-MP702481FHY
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    tdh
  • Uniprot No.：
  • 別名：
    tdh; TK0916; L-threonine 3-dehydrogenase; TDH; EC 1.1.1.103; L-threonine dehydrogenase
  • 種屬：
    Pyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1))
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-350
  • 氨基酸序列
    MAEKMQAIMK TKPAYGAELV EVDVPKPGPG EVLIKVLATS ICGTDLHIYE WNEWAQSRIK PPQIMGHEVA GEVVEVGPGV EDLQVGDYIS VETHIVCGKC YACKHNRYHV CQNTKIFGVD MDGVFAHYAI VPAKNAWKNP KDMPPEYAAL QEPLGNAVDT VLAGPIAGRS TLITGAGPLG LLGIAVAKAS GAYPVIVSEP SEFRRKLAKK VGADYVVNPF EEDPVKFVMD ITDGAGVEVF LEFSGAPKAL EQGLKAVTPG GRVSLLGLFP REVTIDFNNL IIFKALEVHG ITGRHLWETW YTVSSLIQSG KLNLDPIITH KYKGFDKFEE AFELMRAGKT GKVVFFPHKG
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalyzes the NAD(+)-dependent oxidation of L-threonine to 2-amino-3-ketobutyrate. To a lesser extent, also catalyzes the oxidation of L-serine.
  • 基因功能參考文獻(xiàn)：
    1. Crystallization and preliminary structural studies of L-threonine dehydrogenase from hyperthermophilic archaeon T. kodakaraensis KOD1 are reported and will further our understanding of the enzyme's stability at extremes of temperature and pH. PMID: 18765916
    2. TDH expressed in E coli, purified, characterized. Sequence aligned. Activity highest at pH 12, 90C; dependent on divalent cations (e.g., Zn). At 50C, Km is 1.6mM for L-threonine and 0.028mM for NAD+. Half-life is > 2h at 85C, 24min in boiling water. PMID: 19307254
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Zinc-containing alcohol dehydrogenase family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: tko:TK0916

    STRING: 69014.TK0916



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