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  4. Recombinant Pseudomonas aeruginosa Chaperone protein DnaK (dnaK), partial

Recombinant Pseudomonas aeruginosa Chaperone protein DnaK (dnaK), partial

  • 中文名稱：
    銅綠假單胞菌dnaK重組蛋白
  • 貨號(hào)：
    CSB-YP875861EZX
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    銅綠假單胞菌dnaK重組蛋白
  • 貨號(hào)：
    CSB-EP875861EZX
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    銅綠假單胞菌dnaK重組蛋白
  • 貨號(hào)：
    CSB-EP875861EZX-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    銅綠假單胞菌dnaK重組蛋白
  • 貨號(hào)：
    CSB-BP875861EZX
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    銅綠假單胞菌dnaK重組蛋白
  • 貨號(hào)：
    CSB-MP875861EZX
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    dnaK
  • Uniprot No.：
  • 別名：
    dnaK; PA4761Chaperone protein DnaK; HSP70; Heat shock 70 kDa protein; Heat shock protein 70
  • 種屬：
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

問答及客戶評(píng)論

■ 常見問題解答
Q:

Please provide the following information regarding CSB-EP875861EZX:
1.Sequence
2.Tag type
3.Tag position
4.Pricing, sizes and formats

1)Can you please confirm the bacteria species of the protein?
2)How low is the endotoxin removal if we request endotoxin removal service?
3)Also, we are interested in 0.1mg or 0.05mg of this product (lyophilization).

A:
Thanks for your inquiry.
Recombinant Pseudomonas aeruginosa Chaperone protein DnaK(dnaK) ,partial
CSB-EP875861EZX >> E.coli
Expression Region: 380-637aa; Fragment at the C-terminal.
Tag information:Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

AVLAGDVKDVLLLDVTPLTLGIETLGGVMTGLIEKNTTIPTKKSQVFSTADDNQGAVTIHVLQGERKQAAQNKSLGKFDLADIPPAPRGVPQIEVTFDIDANGILHVSAKDKATGKQQSIVIKASSGLSEDEIQQMVRDAEANAEEDRKFEELAAARNQGDALVHATRKMITEAGDKATAEDKATIEKALGELEAAVKGDDKAEIEAKMNALSQASTPLAQKMYAEQAQQGEDAPQGEQAKAADDVVDAEFEEVKDNK


Reference: https://www.uniprot.org/uniprot/Q9HV43
①The bacteria species of the protein is Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
②Please remark your requirement when you place the order if you require for endotoxin removal, we can only guarantee that the final endotoxin level will be less than <1.0 EU per 1μg of the protein by the LAL method.
③We can provide lyophilization service for free, and probably 6% trehalose will be added as protective agent during the lyophilization,
lead time will be extended for 3 working days.

靶點(diǎn)詳情

  • 功能：
    Acts as a chaperone.
  • 基因功能參考文獻(xiàn)：
    1. Inactivation of dnaK caused significant repression of bacterial penetration through Caco-2 cell monolayers, with decreased swimming, swarming and twitching motilities. PMID: 28758636
  • 蛋白家族：
    Heat shock protein 70 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: pae:PA4761

    STRING: 208964.PA4761



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