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  4. Recombinant Pseudomonas aeruginosa Beta-lactamase (ampC)

Recombinant Pseudomonas aeruginosa Beta-lactamase (ampC)

  • 中文名稱：
    Recombinant Pseudomonas aeruginosa Beta-lactamase(ampC)
  • 貨號：
    CSB-YP326492EZX
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Pseudomonas aeruginosa Beta-lactamase(ampC)
  • 貨號：
    CSB-EP326492EZX-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Pseudomonas aeruginosa Beta-lactamase(ampC)
  • 貨號：
    CSB-BP326492EZX
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Pseudomonas aeruginosa Beta-lactamase(ampC)
  • 貨號：
    CSB-MP326492EZX
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ampC
  • Uniprot No.：
  • 別名：
    ampC; PA4110Beta-lactamase; EC 3.5.2.6; Cephalosporinase
  • 種屬：
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    27-397
  • 氨基酸序列
    GEAP ADRLKALVDA AVQPVMKAND IPGLAVAISL KGEPHYFSYG LASKEDGRRV TPETLFEIGS VSKTFTATLA GYALTQDKMR LDDRASQHWP ALQGSRFDGI SLLDLATYTA GGLPLQFPDS VQKDQAQIRD YYRQWQPTYA PGSQRLYSNP SIGLFGYLAA RSLGQPFERL MEQQVFPALG LEQTHLDVPE AALAQYAQGY GKDDRPLRVG PGPLDAEGYG VKTSAADLLR FVDANLHPER LDRPWAQALD ATHRGYYKVG DMTQGLGWEA YDWPISLKRL QAGNSTPMAL QPHRIARLPA PQALEGQRLL NKTGSTNGFG AYVAFVPGRD LGLVILANRN YPNAERVKIA YAILSGLEQQ GKVPLKR
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

問答及客戶評論

■ 常見問題解答
Q:

Would you happen to know the activity of this beta lactamase against nitrocefin?

A:
Thanks for your inquiry. I'm afraid we haven't detect the activity of this beta lactamase against nitrocefin. Pls refer to: https://www.ncbi.nlm.nih.gov/pubmed/26142222

靶點詳情

  • 基因功能參考文獻：
    1. Among several acquired beta-lactamase enzymes, the blaPER-1 and blaVEB-1, although produced less frequently, have clinical importance because of conferring resistance to oxyimino beta-lactams. PMID: 26944896
    2. Changes have been identified within AmpD responsible for derepression of ampC and conferring ceftazidime resistance. PMID: 27067331
    3. Pseudomonas aeruginosa is able to adapt to efficacious beta-lactams, including the newer cephalosporin ceftolozane, through a variety of mutations affecting its intrinsic beta-lactamase, AmpC. PMID: 26248364
    4. Overexpression of the ampC gene was observed in carbapenem-resistant P. aeruginosa isolates, but no carbapenem-susceptible isolates overexpressed the ampC gene. PMID: 22633564
  • 亞細胞定位：
    Periplasm.
  • 蛋白家族：
    Class-C beta-lactamase family
  • 數據庫鏈接：

    KEGG: pae:PA4110

    STRING: 208964.PA4110



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