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  4. Recombinant Protein lin-28 (lin-28)

Recombinant Protein lin-28 (lin-28)

  • 中文名稱：
    秀麗隱桿線蟲lin-28重組蛋白
  • 貨號：
    CSB-YP308988CXY
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲lin-28重組蛋白
  • 貨號：
    CSB-EP308988CXY
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲lin-28重組蛋白
  • 貨號：
    CSB-EP308988CXY-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲lin-28重組蛋白
  • 貨號：
    CSB-BP308988CXY
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲lin-28重組蛋白
  • 貨號：
    CSB-MP308988CXY
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    lin-28
  • Uniprot No.：
  • 別名：
    lin-28; F02E9.2Protein lin-28; Abnormal cell lineage protein 28
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-227
  • 氨基酸序列
    MSTVVSEGRN DGNNRYSPQD EVEDRLPDVV DNRLTENMRV PSFERLPSPT PRYFGSCKWF NVSKGYGFVI DDITGEDLFV HQSNLNMQGF RSLDEGERVS YYIQERSNGK GREAYAVSGE VEGQGLKGSR IHPLGRKKAV SLRCFRCGKF ATHKAKSCPN VKTDAKVCYT CGSEEHVSSI CPERRRKHRP EQVAAEEAEA ARMAAEKSSP TTSDDDIREK NSNSSDE
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Heterochronic protein which controls the choice of stage specific cell fates. Regulates the timing of the second larval stage events (L2 events) in the hypodermis. May negatively regulate the larval to adult transition via the suppression of the microRNA (miRNA) let-7 during L3. Through this regulatory role, controls the timing of the sexual maturation of the nervous system. Also has a role in the fox-1-sex-1-mediated determination of sexual fate. Plays a role in governing the developmental timing of male tail tip morphogenesis. Plays a role in controlling the seam cell number during larval stages. Plays a role in vulval development.
  • 基因功能參考文獻(xiàn)：
    1. show that CED-3 caspase and the E3 ubiquitin ligase UBR-1 form a complex that couples their in vivo activities, allowing for recognition and rapid degradation of LIN-28 and thus facilitating a switch in developmental programs PMID: 28602583
    2. LEP-2 regulates LIN-28 stability to promote the juvenile-to-adult transition in Caenorhabditis elegans PMID: 26811380
    3. LIN-28 regulates the expression of the let-7 microRNA by binding its primary transcript in a previously unknown region, revealing a novel regulatory mechanism. PMID: 25805859
    4. lin-28's two-step mechanism may be an essential feature of its evolutionarily conserved role in cell fate succession PMID: 22457637
    5. LIN28 can directly inhibit let-7g biogenesis at the Dicer processing step PMID: 21815640
    6. Lin-28 binds endogenous primary let-7 microRNA transcripts co-transcriptionally. PMID: 21297634
    7. lin-28, the RNA binding protein, is required for the correct timing of let-7 RNA and let-7:gfp expression. PMID: 12871707
    8. These results suggest that lin-28 expression is regulated by multiple independent mechanisms. PMID: 17139256
    9. LIN-28 and the poly(U) polymerase PUP-2 regulate let-7 microRNA processing in Caenorhabditis elegans PMID: 19713957

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Lin-28 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: cel:CELE_F02E9.2

    STRING: 6239.F02E9.2a

    UniGene: Cel.22515



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