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  4. Recombinant Probable serine/threonine-protein kinase zyg-1 (zyg-1), partial

Recombinant Probable serine/threonine-protein kinase zyg-1 (zyg-1), partial

  • 中文名稱：
    秀麗隱桿線蟲zyg-1重組蛋白
  • 貨號：
    CSB-YP872406CXY
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲zyg-1重組蛋白
  • 貨號：
    CSB-EP872406CXY
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲zyg-1重組蛋白
  • 貨號：
    CSB-EP872406CXY-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲zyg-1重組蛋白
  • 貨號：
    CSB-BP872406CXY
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲zyg-1重組蛋白
  • 貨號：
    CSB-MP872406CXY
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    zyg-1
  • Uniprot No.：
  • 別名：
    zyg-1; F59E12.2Probable serine/threonine-protein kinase zyg-1; EC 2.7.11.1; Zygote defective protein 1
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Protein kinase that plays a central role in centrosome duplication, control of centrosome size, spindle formation and nuclear envelope breakdown during cell divisions. Paternal copy is required to regulate synthesis of daughter centrioles prior to fertilization. Maternal copy regulates centrosome duplication during later cell cycles. Functions upstream of sas-5 and sas-6, and is required for their localization to the centrosome. Its role in nuclear envelope breakdown is mediated by the spindly-like protein spdl-1 and the RZZ complex, which in turn recruits the spindle checkpoint proteins mdf-1 and mdf-2, dynein and dynactin to unattached kinetochores.
  • 基因功能參考文獻：
    1. These findings lead us to conclude that ZYG-1 promotes limited centriole amplification solely during the symmetric division in the C. elegans seam lineage. PMID: 29307730
    2. Structure of the C. elegans ZYG-1 cryptic polo box suggests a conserved mechanism for centriolar docking of Plk4 kinases. PMID: 24980795
    3. Direct interactions of the SAS-6 coiled coil with ZYG-1 and SAS-5 are required to recruit SAS-6 to the mother centriole. PMID: 23673331
    4. Data show that levels of the C. elegans Plk4 homolog ZYG-1 are elevated by impairing proteasome or SCF function, indicating that ZYG-1 is regulated by a conserved mechanism. PMID: 22623721
    5. Data reveal differences in the regulation of mitotic and meiotic centrosome duplication, particularly with regard to ZYG-1 activity, and reveal an important role for centrosomes in spermatid formation. PMID: 20144993
    6. Study demonstrates that the kinase ZYG-1 phosphorylates the coiled-coil protein SAS-6 at serine 123 in vitro; this phosphorylation event is crucial for centriole formation in vivo. PMID: 20059959
    7. Numerous candidate regulators of centrosome duplication and regulatory mechanism involving factors that tether the centrosome to the nucleus, have been identified. PMID: 17446307
    8. SZY-20 opposes the Plk4-related kinase ZYG-1 to limit centrosome size PMID: 19081077

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Note=Component of the centrosome (PubMed:11371350). Present at centrioles only immediately before their duplication (PubMed:11371350). Expression at centrosomes increases from prophase to anaphase during mitosis (PubMed:22623721).
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family
  • 數(shù)據(jù)庫鏈接：


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