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  4. Recombinant Pig Potassium-transporting ATPase alpha chain 1 (ATP4A), partial

Recombinant Pig Potassium-transporting ATPase alpha chain 1 (ATP4A), partial

  • 中文名稱：
    Recombinant Pig Potassium-transporting ATPase alpha chain 1(ATP4A) ,partial
  • 貨號：
    CSB-YP002342PI
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Pig Potassium-transporting ATPase alpha chain 1(ATP4A) ,partial
  • 貨號：
    CSB-EP002342PI
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Pig Potassium-transporting ATPase alpha chain 1(ATP4A) ,partial
  • 貨號：
    CSB-EP002342PI-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Pig Potassium-transporting ATPase alpha chain 1(ATP4A) ,partial
  • 貨號：
    CSB-BP002342PI
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Pig Potassium-transporting ATPase alpha chain 1(ATP4A) ,partial
  • 貨號：
    CSB-MP002342PI
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    ATP4APotassium-transporting ATPase alpha chain 1; EC 7.2.2.19; Gastric H(+)/K(+) ATPase subunit alpha; Proton pump
  • 種屬：
    Sus scrofa (Pig)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    The catalytic subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Uses ATP as an energy source to pump H(+) ions to the gastric lumen while transporting K(+) ion from the lumen into the cell. Remarkably generates a million-fold proton gradient across the gastric parietal cell membrane, acidifying the gastric juice down to pH 1. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). The release of the H(+) ion in the stomach lumen is followed by binding of K(+) ion converting the pump conformation back to the E1 state.
  • 基因功能參考文獻：
    1. New cryo-electron microscopic structures of H+,K+-ATPase using a phosphate analog and a specific antagonist, SCH28080. PMID: 25231997
    2. for the Na(+)/K(+)-ATPase, Mg(2+)-vanadate is unable to recruit all the Rb(+)-bound to the Rb(+)-occluded form of the Rb(+)-vanadate-H(+)/K(+)-ATPase complex PMID: 20826127
    3. Results provide strong evidence that the catalytic unit of octaethylene glycol dodecyl ether-solubilized gastric H/K-ATPase is a tetraprotomer. PMID: 16169880
    4. Data describe the functional characteristics of the oligomeric form of the membrane-bound gastric H,K-ATPase. PMID: 16331993
    5. The number of parietal cells explained part of the differences in the expression of the H(+)/K(+)-ATPase gene. PMID: 16614409
    6. structural rearrangements in the H+,K+-ATPase are much smaller than those observed in the related SERCA ATPase PMID: 18276159

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  • 亞細胞定位：
    Apical cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIC subfamily
  • 數據庫鏈接：


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