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  4. Recombinant Pan troglodytes Tripartite motif-containing protein 5 (TRIM5)

Recombinant Pan troglodytes Tripartite motif-containing protein 5 (TRIM5)

  • 中文名稱：
    平頭肌球蛋白TRIM5重組蛋白
  • 貨號：
    CSB-YP710697EQV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    平頭肌球蛋白TRIM5重組蛋白
  • 貨號：
    CSB-EP710697EQV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    平頭肌球蛋白TRIM5重組蛋白
  • 貨號：
    CSB-EP710697EQV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    平頭肌球蛋白TRIM5重組蛋白
  • 貨號：
    CSB-BP710697EQV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    平頭肌球蛋白TRIM5重組蛋白
  • 貨號：
    CSB-MP710697EQV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    TRIM5
  • Uniprot No.：
  • 別名：
    TRIM5; Tripartite motif-containing protein 5; EC 2.3.2.27; RING-type E3 ubiquitin transferase TRIM5; TRIM5alpha
  • 種屬：
    Pan troglodytes (Chimpanzee)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-493
  • 氨基酸序列
    MASGILVNVK EEVTCPICLE LLTQPLSLDC GHSFCQACLT ANHKKSMLDK GESSCPVCRI SYQPENIRPN RHVANIVEKL REVKLSPEGQ KVDHCAHHGE KLLLFCQEDG KVICWLCERS QEHRGHHTFL TEEVAREYQV KLQAALEMLR QKQQEAEELE ADIREEKASW KTQIQYDKTN VLADFEQLRD ILDWEESNEL QNLEKEEEDI LKSLTKSETE MVQQTQSVRE LISDLERRLQ GSVMELLQGV DGVIKRMENV TLKKPETFPK NQRRVFRAPD LKGMLEVFRE LTDVRRYWVD VTVAPNNISC AVISEDMRQV SSPKPQIIYG ARGTRYQTFM NFNYCTGILG SQSITSGKHY WEVDVSKKSA WILGVCAGFQ PDAMCNIEKN ENYQPKYGYW VIGLEEGVKC SAFQDGSFHT PSAPFIVPLS VIICPDRVGV FLDYEACTVS FFNITNHGSL IYKFSHCSFS QPVFPYLNPR KCGVPMTLCS PSS
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Plays a role in regulating autophagy through activation of autophagy regulator BECN1 by causing its dissociation from its inhibitors BCL2 and TAB2.
  • 基因功能參考文獻：
    1. TRIM5alpha protein functionally resembled human TRIM5alpha, potently restricting infection by N-tropic murine leukemia virus (N-MLV) and moderately restricting human immunodeficiency virus type 1 (HIV-1) infection PMID: 15767395
    2. SPRY domain of TRIM5alpha showed higher nonsynon/synonymous substitution ratios than the non-SPRY domain, indicating that the adaptive evolution of TRIM5alpha might be a strategy developed in defending retrovirus infection during primate evolution. PMID: 16226405
  • 亞細胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    TRIM/RBCC family
  • 數據庫鏈接：


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