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  4. Recombinant Mouse tRNA (cytosine (38)-C (5))-methyltransferase

Recombinant Mouse tRNA (cytosine (38)-C (5))-methyltransferase

  • 中文名稱：
    小鼠Trdmt1重組蛋白
  • 貨號：
    CSB-YP526321MO
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Trdmt1重組蛋白
  • 貨號：
    CSB-EP526321MO
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Trdmt1重組蛋白
  • 貨號：
    CSB-EP526321MO-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Trdmt1重組蛋白
  • 貨號：
    CSB-BP526321MO
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Trdmt1重組蛋白
  • 貨號：
    CSB-MP526321MO
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Trdmt1
  • Uniprot No.：
  • 別名：
    Trdmt1; Dnmt2; Met2; tRNA; cytosine(38)-C(5))-methyltransferase; EC 2.1.1.204; DNA; cytosine-5)-methyltransferase-like protein 2; Dnmt2; DNA methyltransferase homolog MmuIIP; DNA MTase homolog MmuIIP; M.MmuIIP; Met-2
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-415
  • 氨基酸序列
    MEPLRVLELY SGIGGMHHAL RESHIPAHVV AAIDVNTVAN EVYKHNFPHT HLLSKTIEGI SLEDFDKLSF NMILMSPPCQ PFTRIGLQGD MTDPRTTSFL YILDILPRLQ KLPKYILLEN VKGFEVSSTR GLLIQTIEAC GFQYQEFLLS PSSLGIPNSR LRYFLIAKLQ SEPFPFQAPG QILMEFPKIV TVEPQKYAVV EESQPRVQRT GPRICAESSS TQSSGKDTIL FKLETVEERD RKHQQDSDLS VQMLKDFLED GDTDEYLLPP KLLLRYALLL DIVKPTSRRS MCFTKGYGSY IEGTGSVLQA AEDAQIENIY KSLPDLPPEE KIAKLSMLKL RYFTPKEIAN LQGFPPEFGF PEKTTVKQRY RLLGNSLNVH VVAKLLTVLC EGFGNASESC HKMPLILDSN SKILS
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).
  • 基因功能參考文獻：
    1. Dnmt2 may promote lifespan in the control conditions and survival during stress conditions in mouse fibroblasts. PMID: 28177119
    2. Dnmt2 plays an unexpected role for regulation of cardiac growth by modulating activity of the P-TEFb complex. PMID: 27270731
    3. Treatment of aged mice and their derived macrophages with methyltransferase inhibitor (2)-epigallocatechin-3-gallate (EGCG) or specific DNA methyltransferase 2 (DNMT2) siRNA restored the expression of Atg5 and LC3 in vivo and in vitro. PMID: 26909551
    4. Dnmt2 plays an important role in haematopoiesis and define a novel function of C38 tRNA methylation in the discrimination of near-cognate codons, thereby ensuring accurate polypeptide synthesis. PMID: 26271101
    5. these findings uncover a novel function of Dnmt2 in RNA-mediated epigenetic heredity PMID: 23717211
    6. Dnmt2-dependent methylomes lack defined DNA methylation patterns. PMID: 23641003
    7. Steady-state levels of unmethylated tRNAs were substantially reduced, and loss of Dnmt2 and NSun2 was further associated with reduced rates of overall protein synthesis. PMID: 22885326
    8. the role of Dnmt2 in stress granules could represent a primitive cellular defense mechanism against viral infection. PMID: 20864816

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, C5-methyltransferase family
  • 組織特異性：
    Highly expressed in thymus, testis, and at much lower levels in spleen, lung, brain, heart, kidney, liver, skeletal muscle and embryonic stem cells.
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:13434

    STRING: 10090.ENSMUSP00000114572

    UniGene: Mm.6979



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