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  4. Recombinant Mouse Wee1-like protein kinase 2 (Wee2)

Recombinant Mouse Wee1-like protein kinase 2 (Wee2)

  • 中文名稱：
    小鼠Wee2重組蛋白
  • 貨號：
    CSB-YP727742MO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Wee2重組蛋白
  • 貨號：
    CSB-EP727742MO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Wee2重組蛋白
  • 貨號：
    CSB-EP727742MO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Wee2重組蛋白
  • 貨號：
    CSB-BP727742MO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Wee2重組蛋白
  • 貨號：
    CSB-MP727742MO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Wee2; Wee1b; Wee1-like protein kinase 2; EC 2.7.10.2; Wee1-like protein kinase 1B; Wee1B kinase; mWee1B
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-555
  • 氨基酸序列
    MADTETDQGL NKKLSFSFCE EDTESEGQMT AQDIGGAQSQ KPGREESEEL EGPVPPTRDE LHTSLSRDKE SPGPDLWKSV SGPPKCPETP VLHSKSKLPV PTNFSTPKNS LGQPEISLLE KLSSRSSRHM RLTPASLMDE KTSLSLVNIN PFTPETYRKL LLQSKGKRKT RDYLEETGEE ESKSVQWLPA KRSILQETNM ASRYEKEFFE IEKIGVGEFG TVYKCIKRLD GCIYAIKRSA KSFSGLSNEL DLHEVYAHAV LGHHPHVVRY YSSWIEDDHV VIQNEYCNGG SLQAAISENT ASNNHFQEPK LKDILLQISL GLKYIHNSGM VHLDIKPSNI FICHKMQCDS PVGPEEAESE ADWFLNASVM YKIGDLGHAT SISKPKVEEG DTRFLANEIL QENYQHLPKA DIFALGLTIA VAAGAESLPI NGDMWHHIRK GNFPEISQEL SDDFYGLLKN MIHPAPKERP SAAALARSRI LWPFLEKTDE LQKQLNLEKS KTATLKRELK KARHIQTPQR EVHHCYYQIC KGSRHLLVGG RRKAPSSFTR GTSSV
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.
  • 基因功能參考文獻：
    1. WEE1B acts as a direct downstream substrate of protein kinase A (PKA) and that Ser 15 of WEE1B is a potential PKA phosphorylation target in the G2/M transition of mouse embryos. PMID: 23616086
    2. findings demonstrate that exit from metaphase requires not only a proteolytic degradation of cyclin B but also the inhibitory phosphorylation of Cdc2 by Wee1B PMID: 21454751
    3. Wee1B is a key maturation-promoting-factor inhibitory kinase in mouse oocytes, functions downstream of PKA, and is required for maintaining meiotic arrest. PMID: 16169490
  • 亞細胞定位：
    Cytoplasm. Nucleus. Note=Localizes mainly in the nucleus. Exported from the nucleus to the cytoplasm before germinal vesicle breakdown (GVBD), allowing meiosis resumption.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, WEE1 subfamily
  • 組織特異性：
    Ovary-specific.
  • 數據庫鏈接：


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