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  4. Recombinant Mouse Ubiquitin-fold modifier 1 (Ufm1)

Recombinant Mouse Ubiquitin-fold modifier 1 (Ufm1)

  • 中文名稱：
    小鼠Ufm1重組蛋白
  • 貨號：
    CSB-YP025560MO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Ufm1重組蛋白
  • 貨號：
    CSB-EP025560MO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Ufm1重組蛋白
  • 貨號：
    CSB-EP025560MO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Ufm1重組蛋白
  • 貨號：
    CSB-BP025560MO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Ufm1重組蛋白
  • 貨號：
    CSB-MP025560MO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Ufm1
  • Uniprot No.：
  • 別名：
    Ufm1Ubiquitin-fold modifier 1
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full length protein
  • 表達區域：
    1-83
  • 氨基酸序列
    MSKVSFKITLTSDPRLPYKVLSVPESTPFTAVLKFAAEEFKVPAATSAIITNDGIGINPA QTAGNVFLKHGSELRIIPRDRVG
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Ubiquitin-like modifier which can be covalently attached via an isopeptide bond to lysine residues of substrate proteins as a monomer or a lysine-linked polymer. The so-called ufmylation, requires the UFM1-activating E1 enzyme UBA5, the UFM1-conjugating E2 enzyme UFC1, and the UFM1-ligase E3 enzyme UFL1. Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress. Ufmylation of TRIP4 regulates nuclear receptors-mediated transcription.
  • 基因功能參考文獻：
    1. Ufmylation may participate in regulation of the vascular smooth muscle cells phenotypic switch and endothelial cell injury, which may help in the understanding of vascular remodeling. PMID: 29461087
    2. CNS-specific knockout of Ufm1 in mice causes neonatal death accompanied by microcephaly and apoptosis in specific neurons, further suggesting that the UFM1 system is essential for CNS development and function PMID: 27545674
    3. High UFM1 expression is associated with Atherosclerosis. PMID: 26040753
    4. transcription downregulation of the Ufm1 and FAT10 conjugation system in liver Mallory-Denk bodies formation PMID: 24893112
    5. These data suggest that UFM1-UFBP1 participate in preventing ER stress-induced apoptosis in protein secretory cells. PMID: 21494687
    6. A novel type of E3 ligase for the Ufm1 conjugation system. PMID: 20018847
    7. A novel ubiquitin fold modifier (Ufm1) not been previously associated with ER stress and not found to be induced under any condition was found to be upregulated in the hearts of MCP mice (transgenic mice that express MCP-1 specifically in the heart). PMID: 16617122
    8. loop between beta3 and alpha2 in addition to the C-terminal region of Ufm1 plays a role in binding to UfSP1 PMID: 18321862

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  • 亞細胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    UFM1 family
  • 組織特異性：
    Widely expressed with higher expression in secretory tissues (at protein level).
  • 數據庫鏈接：


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