Recombinant Mouse Tryptophan 5-hydroxylase 1 (Tph1)

^{In Stock}

中文名稱：

小鼠Tph1重組蛋白
貨號：

CSB-EP024100MO
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Tph1
Uniprot No.：

P17532
別名：

Tph1; Tph; Tryptophan 5-hydroxylase 1; EC 1.14.16.4; Tryptophan 5-monooxygenase 1
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length
來源：

E.coli
分子量：

67.3kDa
表達區域：

1-447aa
氨基酸序列

MIEDNKENKENKDHSSERGRVTLIFSLENEVGGLIKVLKIFQENHVSLLHIESRKSKQRNSEFEIFVDCDISREQLNDIFPLLKSHATVLSVDSPDQLTAKEDVMETVPWFPKKISDLDFCANRVLLYGSELDADHPGFKDNVYRRRRKYFAELAMNYKHGDPIPKIEFTEEEIKTWGTIFRELNKLYPTHACREYLRNLPLLSKYCGYREDNIPQLEDVSNFLKERTGFSIRPVAGYLSPRDFLSGLAFRVFHCTQYVRHSSDPLYTPEPDTCHELLGHVPLLAEPSFAQFSQEIGLASLGASEETVQKLATCYFFTVEFGLCKQDGQLRVFGAGLLSSISELKHALSGHAKVKPFDPKIACKQECLITSFQDVYFVSESFEDAKEKMREFAKTVKRPFGLKYNPYTQSVQVLRDTKSITSAMNELRYDLDVISDALARVTRWPSV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-SUMO-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Oxidizes L-tryptophan to 5-hydroxy-l-tryptophan in the rate-determining step of serotonin biosynthesis.
基因功能參考文獻：
1. Both gut-specific Tph1 knockout mice and liver-specific Htr2a knockout mice are resistant to high fat diet-induced hepatic steatosis, without affecting systemic energy homeostasis. PMID: 30446669
2. Data suggest that central melanocortin-4 receptor (MC4-R) and tryptophan hydroxylase (TPH) are involved in the efferent neuronal control of the kidneys. PMID: 27626491
3. Prolactin-induced Tph1 expression. PMID: 26136513
4. Tph1-deficient mice fed a high-fat diet (HFD) are protected from obesity, insulin resistance and nonalcoholic fatty liver disease (NAFLD) while exhibiting greater energy expenditure by brown adipose tissue. PMID: 25485911
5. Lack of tryptophan hydroxylase-1 in mice results in gait abnormalities. PMID: 23516593
6. ZBP-89 directly binds to the Tph1 promoter, which is required for its basal and butyrate-inducible expression. PMID: 23395646
7. TPH1 deficiency or inhibition reduces allergic airway inflammation. Platelet-derived 5-HT is pivotal in AAI and lack of 5-HT leads to impaired Th2 priming capacity of bone marrow dendritic cells. PMID: 23328530
8. Data indicate that tryptophan hydroxylase (Tph)1-deficient mice, lacking non-neuronal serotonin, showed mild leukocytosis. PMID: 23243271
9. Data show that Tph-1 (tryptophan hydroxylase-1) is a potent regulator of immunity. PMID: 23008335
10. Parallel to Tph1 mRNA and protein induction, islet serotonin content increased to a peak level that was 200-fold higher than basal. Interestingly, only a subpopulation of the beta cells was serotonin-positive in vitro and in vivo. PMID: 20938637
11. nonneuronal tph1 gene has a role in cardiac function with peripheral serotonin PMID: 14597720
12. Review. TPH1 is expressed in the pineal gland and the enterochromaffin cells. Loss of TPH1 gene expression, and thus of peripheral 5-HT, leads to a cardiac dysfunction phenotype. PMID: 15146950
13. estrogen receptor-beta is responsible for mediating estrogen regulated tryptophan hydroxylase-1 expression in the murine dorsal raphe nucleus PMID: 15820717
14. TPH1 is expressed preferentially during late developmental brain stages. Low raphe nuclei 5-HT contents were seen during development in NZW & SWR mice with common functional polymorphisms in TPH1. TPH1 may influence behavior later in life. PMID: 16407550
15. TPH1 is the predominant form expressed in pineal gland and with a molecular weight of 51 kDa. PMID: 16581041
16. Tryptophan hydroxylase 1 and peripheral serotonin play an essential role in the development of hypoxia-induced elevations in pulmonary pressures and hypoxia-induced pulmonary vascular remodeling. PMID: 17130306
17. Tph1 knockout mice used to show that serotonin plays a role in the pathogenesis of steatohepatitis. PMID: 17681180
18. Cardiomyopathy is more severe in adult Tph1 knockout mice born to homozygous mothers than to heterozygous mothers. PMID: 18267982
19. In a genetic model of serotonin deficiency (Tph1(-/-)) in mice, serotonin shown to be crucial for the growth of s.c. colon cancer allografts in vivo. PMID: 18593914
20. substantially reduced peripheral 5-HT levels in Tph1/Tph2 double knockout, but not TPH2KO mice PMID: 18923670
21. In mice no significant TPH1 expression was detected in the brain. PMID: 19181488
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蛋白家族：

Biopterin-dependent aromatic amino acid hydroxylase family
數據庫鏈接：

KEGG: mmu:21990

STRING: 10090.ENSMUSP00000037752

UniGene: Mm.248684