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  4. Recombinant Mouse Sphingomyelin phosphodiesterase 2 (Smpd2), partial

Recombinant Mouse Sphingomyelin phosphodiesterase 2 (Smpd2), partial

  • 中文名稱：
    小鼠Smpd2重組蛋白
  • 貨號：
    CSB-YP021846MO1
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Smpd2重組蛋白
  • 貨號：
    CSB-EP021846MO1
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Smpd2重組蛋白
  • 貨號：
    CSB-EP021846MO1-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Smpd2重組蛋白
  • 貨號：
    CSB-BP021846MO1
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Smpd2重組蛋白
  • 貨號：
    CSB-MP021846MO1
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Smpd2
  • Uniprot No.：
  • 別名：
    Smpd2; Sphingomyelin phosphodiesterase 2; Lyso-platelet-activating factor-phospholipase C; Lyso-PAF-PLC; Neutral sphingomyelinase; N-SMase; nSMase
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Hydrolyze 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor).
  • 基因功能參考文獻：
    1. nSMase2 has a role in ceramide generation, aberrant apoptosis, and lung injury under cigarette smoke exposure PMID: 20448054
    2. Neutral sphingomyelinase 1 deficiency in the mouse causes no lipid storage disease. (nSMase 1) PMID: 11997500
    3. NSMase-2 could be involved in IL-1beta-induced JNK activation in hepatocytes PMID: 15059969
    4. Nuclear sphingomyelin-synthase and protein kinase C delta are expressed in melanoma cells PMID: 15907783
    5. Peptidoglycan activates the nSMase/ceramide pathway to induce MKK3/6/p38 MAPK activation, which in turn initiates NF-kappaB activation and ultimately induces COX-2 expression in macrophages. PMID: 19531467

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  • 亞細胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Neutral sphingomyelinase family
  • 組織特異性：
    Although widely expressed in all tissues examined, except the spleen, high enzymatic activity occurs only in the brain.
  • 數據庫鏈接：

    KEGG: mmu:20598

    STRING: 10090.ENSMUSP00000019965

    UniGene: Mm.953



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