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  4. Recombinant Mouse Serine/threonine-protein kinase Nek7 (Nek7)

Recombinant Mouse Serine/threonine-protein kinase Nek7 (Nek7)

  • 中文名稱：
    小鼠Nek7重組蛋白
  • 貨號：
    CSB-YP872141MO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Nek7重組蛋白
  • 貨號：
    CSB-EP872141MO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Nek7重組蛋白
  • 貨號：
    CSB-EP872141MO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Nek7重組蛋白
  • 貨號：
    CSB-BP872141MO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Nek7重組蛋白
  • 貨號：
    CSB-MP872141MO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Nek7; Serine/threonine-protein kinase Nek7; EC 2.7.11.1; Never in mitosis A-related kinase 7; NimA-related protein kinase 7
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-302
  • 氨基酸序列
    MDEQSQGMQG PPVTQFQPQK ALRPDMGYNT LANFRIEKKI GRGQFSEVYR ASCLLDGVPV ALKKVQIFDL MDAKARADCI KEIDLLKQLN HPNVIKYYAS FIEDNELNIV LELADAGDLS RMIKHFKKQK RLIPERTVWK YFVQLCSALD HMHSRRVMHR DIKPANVFIT ATGVVKLGDL GLGRFFSSKT TAAHSLVGTP YYMSPERIHE NGYNFKSDIW SLGCLLYEMA ALQSPFYGDK MNLYSLCKKI EQCDYPPLPS DHYSEELRQL VNICINPDPE KRPDIAYVYD VAKRMHACTA ST
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1. Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression.
  • 基因功能參考文獻：
    1. NEK7 regulates dendrite morphogenesis in vitro and in vivo. NEK7 kinase activity is required for dendrite growth and branching, as well as spine formation and morphology. NEK7 regulates these processes in part through phosphorylation of the kinesin Eg5/KIF11, promoting its accumulation on microtubules in distal dendrites. PMID: 29899413
    2. NEK7 serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division. PMID: 26642356
    3. murine macrophages deficient in Nek7 displayed a largely blunted Nlrp3 inflammasome response. PMID: 26553871
    4. NEK7 is an essential protein that acts downstream of potassium efflux to mediate NLRP3 inflammasome assembly and activation PMID: 26814970
    5. results confirm Nek7 as a regulator of cell division, and reveal it as an essential component for mammalian growth and survival PMID: 20473324
    6. During early embryogenesis nek7 is expressed in the site of decidual reaction. Later in embryogenesis, nek7 is highly expressed in the dorsal thalamus. PMID: 11744387

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  • 亞細胞定位：
    Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily
  • 數據庫鏈接：


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