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  4. Recombinant Mouse Protein pitchfork (Pifo)

Recombinant Mouse Protein pitchfork (Pifo)

  • 中文名稱：
    小鼠Pifo重組蛋白
  • 貨號：
    CSB-YP872026MO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Pifo重組蛋白
  • 貨號：
    CSB-EP872026MO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Pifo重組蛋白
  • 貨號：
    CSB-EP872026MO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Pifo重組蛋白
  • 貨號：
    CSB-BP872026MO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Pifo重組蛋白
  • 貨號：
    CSB-MP872026MO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Pifo
  • Uniprot No.：
  • 別名：
    Pifo; Protein pitchfork
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-207
  • 氨基酸序列
    MNTEEIPVAP PLRGVTPALQ WKVNNYSFGT RQARKLFPHY HPPTWLGNLY LPLRGMPHTG PGCYAAATDW NGLAYNLSKV PTSTKGYAIG ARTAVRFKPI SKDVTPYPGM YQKVDTLSEK HKKSFAPFNI LMPRFRSAAK GDSYPGPGTY NPEMKSVPKV TWPMKFGSPD WSQVPCLEKR TLKAELSADK DFRKHRSRVA YFSLYYQ
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    During primary cilia disassembly, involved in cilia disassembly. Required specifically to control cilia retraction as well as the liberation and duplication of the basal body/centrosome. May act by stimulating AURKA activity at the basal body in a cell cycle-dependent manner.
  • 基因功能參考文獻：
    1. The proper expression levels of Pitchfork might play a pivotal role in normal secondary palate morphogenesis. PMID: 25080064
    2. 1700027A23Rik is most abundantly expressed in tissues rich in highly ciliated cells, such as olfactory sensory neurons, and is predicted to be important to cilia. PMID: 17971504
    3. Pitchfork (Pifo) associates with ciliary targeting complexes and accumulates at the basal body during cilia disassembly. Haploinsufficiency causes a unique node cilia duplication phenotype, left-right asymmetry defects, and heart failure. PMID: 20643351
  • 亞細胞定位：
    [Isoform 1]: Golgi apparatus, Golgi stack. Golgi apparatus, trans-Golgi network.; [Isoform 2]: Nucleus.; Cytoplasm. Cytoplasmic vesicle. Note=Accumulates specifically at the basal body and ciliary necklace during the early steps of cilia assembly and disassembly, when structural, functional and regulatory proteins are delivered to cilia. At S phase, accumulates in vesicles and declines during mitosis. In node pit cells, found close to the ciliary membrane along the axoneme. In spermatocytes, localizes to particles along the stabilized microtubules of tails.
  • 組織特異性：
    Expressed in tissues rich in ciliated cells, such as lung, kidney, vas deferens and testis. Both isoforms 1 and 2 are expressed in testis.
  • 數據庫鏈接：


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